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PMID: 1656218 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of the yeast CYT1 gene encoding cytochrome c1 by HAP1 and HAP2/3/4.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 4934-42

Schneider JC, Guarente L

Abstract

Mitochondrial biogenesis requires the coordinate induction of hundreds of genes that reside in the nucleus. We describe here a study of the regulation of the nuclear-encoded cytochrome c1 of the b-c1 complex. Unlike cytochrome c, which is encoded by two genes, CYC1 and CYC7, c1 is encoded by a single gene, CYT1. The regulatory region of the CYT1 promoter contains binding sites for the HAP1 and HAP2/3/4 transactivators that regulate CYC1. The binding of HAP1 to the CYT1 element was studied in detail and found to differ in two important respects from binding to the CYC1 element. First, while CYC1 contains two sites that bind HAP1 cooperatively, CYT1 has a single high-affinity site. Second, while the CYT1 site and the stronger HAP1-binding site of CYC1 share a large block of homology, the HAP1 footprints at these sites are offset by several nucleotides. We discuss how these differences in HAP1 binding might relate to the difference in the biology of cytochrome c and cytochrome c1.

Related Genes
CYT1
MeSH Terms
Base Sequence Binding Sites/genetics CCAAT-Binding Factor Cytochrome c Group/genetics Cytochromes c1/genetics DNA Mutational Analysis DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal/physiology Kinetics Molecular Sequence Data Promoter Regions, Genetic/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
CCAAT-Binding Factor Cytochrome c Group DNA-Binding Proteins Fungal Proteins HAP1 protein, S cerevisiae HAP4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Cytochromes c1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schneider J C
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139-4307.
Guarente L
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.4934-4942.1991
Published
1991-10-00
Pages
4934-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361470
Subset
IM
Grants
NIGMS NIH HHS · GM30454 · United States
Databases
GENBANK
M64827, M73024, M73025, M73026, M73027, S43616, S43620, S57227, X58857, X58858
External Links
PubMed source DOI: 10.1128/mcb.11.10.4934-4942.1991 PMC Full Text View journal details
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