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PMID: 2832731 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site.

Molecular and cellular biology ·Vol. 8 ·No. 2 ·1988-02-00 ·Pages 647-54

Forsburg SL, Guarente L

Abstract

We analyzed upstream activation sequence 2 (UAS2), one of two independent UAS elements in the CYC1 gene of Saccharomyces cerevisiae. Deletions and linker scanning mutations across the 87 base pairs previously defined as UAS2 showed two separate functional elements required for full activity. Region 1, from -230 to -200, contains the principal activation site and responds to the trans-acting regulatory loci HAP2 and HAP3. A portion of region 1 is homologous to two other HAP2-HAP3-responsive UASs and includes the G----A transition mutation UP1, which increases UAS2 activity. This consensus sequence TNATTGGT bears striking similarity to several CAAT box sequences of higher cells. Region 2, from -192 to -178, substantially enhances the activity of region 1, yet has little activity by itself. These regions bind distinct proteins found in crudely fractionated yeast extracts.

MeSH Terms
Base Sequence Chromosome Deletion Cytochrome c Group/genetics Cytochromes c Gene Expression Regulation Genes Genes, Regulator Plasmids Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
CYC1 protein, S cerevisiae Cytochrome c Group Saccharomyces cerevisiae Proteins Cytochromes c
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Forsburg S L
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Guarente L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.2.647-654.1988
Published
1988-02-00
Pages
647-54
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363190
Subset
IM
Grants
NIGMS NIH HHS · 5 ROI GM30454-05 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.2.647-654.1988 PMC Full Text View journal details
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