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PMID: 15466488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis.

Genes & development ·Vol. 18 ·No. 19 ·2004-10-01 ·Pages 2368-79

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS

Abstract

Higher plants undergo a transition from a juvenile to an adult phase of vegetative development prior to flowering. Screens for mutants that undergo this transition precociously produced alleles of two genes required for posttranscriptional gene silencing (PTGS)--SUPPRESSOR OF GENE SILENCING3 (SGS3) and SUPPRESSOR OF GENE SILENCING2(SGS2)/SILENCING DEFECTIVE1 (SDE1)/RNA-DEPENDENT POLYMERASE6 (RDR6). Loss-of-function mutations in these genes have a phenotype similar to that of mutations in the Argonaute gene ZIPPY (ZIP). Epistasis analysis suggests that ZIP, SGS3, SGS2/SDE1/RDR6, and the putative miRNA export receptor, HASTY (HST), operate in the same pathway(s). Microarray analysis revealed a small number of genes whose mRNA is increased in ZIP, SGS3, and SGS2/SDE1/RDR6 mutants, as well as genes that are up-regulated in SGS3 and SGS2/SDE1/RDR6 mutants, but not in ZIP mutants. One of these latter genes (At5g18040) is silenced posttranscriptionally in trans by the sRNA255 family of endogenous, noncoding, small interfering RNAs (siRNAs). The increase in At5g18040 mRNA in SGS3 and SGS2/SDE1/RDR6 mutants is attributable to the absence of sRNA255-like siRNAs in these mutants. These results demonstrate a role for endogenous siRNAs in the regulation of gene expression, and suggest that PTGS plays a central role in the temporal control of shoot development in plants.

MeSH Terms
Arabidopsis/genetics,growth & development Arabidopsis Proteins/physiology Base Sequence RNA, Small Interfering Sequence Homology, Nucleic Acid
Chemicals
Arabidopsis Proteins RNA, Small Interfering
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Peragine Angela
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018, USA.
Yoshikawa Manabu
Wu Gang
Albrecht Heidi L
Poethig R Scott
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1231804
Published
2004-10-01
Pages
2368-79
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC522987
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051893 · United States
NIGMS NIH HHS · R01 GM51893 · United States
External Links
PubMed source DOI: 10.1101/gad.1231804 PMC Full Text View journal details
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