Home LiteratureArticle Details
PMID: 3200835 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Translational repression in eukaryotes: partial purification and characterization of a repressor of ferritin mRNA translation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 24 ·1988-12-00 ·Pages 9503-7

Walden WE, Daniels-McQueen S, Brown PH, Gaffield L, Russell DA, Bielser D, Bailey LC, Thach RE

Abstract

Mouse and rabbit ferritin mRNAs translate very poorly in rabbit reticulocyte lysates relative to most other mRNAs. This translational deficiency is not seen in wheat germ lysates, suggesting the presence of an inhibitor in reticulocyte lysate that is specific for ferritin mRNA. A specific repressor of ferritin mRNA translation has been partially purified from rabbit reticulocytes by differential ultracentrifugation, ammonium sulfate fractionation, and chromatography on phosphocellulose, DEAE-cellulose, and Sephacryl S-300. The elution profile from the latter suggests an aggregate molecular mass of approximately 180 kDa for the repressor. The inhibitory activity of this repressor against native ferritin mRNA can be relieved by adding in vitro transcripts of ferritin light-chain RNAs that contain the first 92 nucleotides of the 5' untranslated region. No other sequences appear to be necessary for this effect.

MeSH Terms
Animals Chromatography, DEAE-Cellulose Chromatography, Gel Ferritins/genetics Mice Molecular Weight Protein Biosynthesis RNA, Messenger/metabolism Rabbits Repressor Proteins/isolation & purification Reticulocytes/analysis Transcription Factors/isolation & purification Ultracentrifugation
Chemicals
RNA, Messenger Repressor Proteins Transcription Factors Ferritins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Walden W E
Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago 60612.
Daniels-McQueen S
Brown P H
Gaffield L
Russell D A
Bielser D
Bailey L C
Thach R E
References (26)
26 references, click to expand
  1. Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms.
    Annu Rev Biochem. 1987;56:289-315 PMID: 3304136
  2. Translational control of gene expression in a normal fibroblast. Characterization of a subclass of mRNAs with unusual kinetic properties.
    Biochemistry. 1986 Apr 22;25(8):2033-41 PMID: 3707930
  3. Conservation of ferritin heavy subunit gene structure: implications for the regulation of ferritin gene expression.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7438-42 PMID: 3478702
  4. Iron regulates ferritin mRNA translation through a segment of its 5' untranslated region.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8478-82 PMID: 3479802
  5. Identification of the iron-responsive element for the translational regulation of human ferritin mRNA.
    Science. 1987 Dec 11;238(4833):1570-3 PMID: 3685996
  6. The importance of the 3'-untranslated region in the translational control of ferritin mRNA.
    J Biol Chem. 1988 Mar 5;263(7):3071-4 PMID: 2449437
  7. Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2171-5 PMID: 3127826
  8. Procedures for enhancing the utility of the metallothionein promoter for the regulated expression of downstream open reading frames.
    Gene. 1987;61(3):317-27 PMID: 3446577
  9. Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation.
    Science. 1988 May 13;240(4854):924-8 PMID: 2452485
  10. Discriminatory interaction of purified eukaryotic initiation factors 4F plus 4A with the 5' ends of reovirus messenger RNAs.
    J Biol Chem. 1988 May 25;263(15):7266-76 PMID: 3366779
  11. Nucleotide sequence of cDNA encoding rabbit ferritin L chain.
    Nucleic Acids Res. 1988 Aug 11;16(15):7741 PMID: 3412911
  12. Cap accessibility correlates with the initiation efficiency of alfalfa mosaic virus RNAs.
    Eur J Biochem. 1985 Mar 15;147(3):549-52 PMID: 2983983
  13. Regulation of synthesis and turnover of ferritin in rat liver.
    J Biol Chem. 1966 Aug 10;241(15):3630-7 PMID: 5919688
  14. On the mechanism of iron-induced synthesis of apoferritin in HeLa cells.
    J Biol Chem. 1969 Jul 25;244(14):3847-54 PMID: 5805400
  15. Novel mechanism for translational control in regulation of ferritin synthesis by iron.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):857-61 PMID: 1083028
  16. Translational control of protein synthesis.
    Annu Rev Biochem. 1976;45:39-72 PMID: 786155
  17. Ferritin: structure, biosynthesis, and role in iron metabolism.
    Physiol Rev. 1978 Apr;58(2):317-96 PMID: 347470
  18. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  19. Iron transport and storage proteins.
    Annu Rev Biochem. 1980;49:357-93 PMID: 6996567
  20. Translational control of ferritin synthesis by iron in embryonic reticulocytes of the bullfrog.
    J Biol Chem. 1982 Dec 10;257(23):14187-91 PMID: 6982898
  21. Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
    Cell. 1985 Mar;40(3):515-26 PMID: 2982496
  22. ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors.
    J Biol Chem. 1985 Jun 25;260(12):7651-8 PMID: 3838990
  23. Both subunits of rat liver ferritin are regulated at a translational level by iron induction.
    Nucleic Acids Res. 1986 Jan 24;14(2):915-27 PMID: 2418417
  24. Cloning of the gene coding for human L apoferritin.
    Nucleic Acids Res. 1986 Apr 11;14(7):2863-76 PMID: 3754330
  25. Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2850-4 PMID: 3458245
  26. A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6730-4 PMID: 3477805
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.24.9503
Published
1988-12-00
Pages
9503-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282781
Subset
IM
Grants
NIAID NIH HHS · AI20484 · United States
NCRR NIH HHS · S07RR5369-26 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.24.9503 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2