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PMID: 3178728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Skeletal muscle mitochondrial beta-oxidation. A study of the products of oxidation of [U-14C]hexadecanoate by h.p.l.c. using continuous on-line radiochemical detection.

The Biochemical journal ·Vol. 253 ·No. 2 ·1988-07-15 ·Pages 541-7

Watmough NJ, Bhuiyan AK, Bartlett K, Sherratt HS, Turnbull DM

Abstract

Well-coupled mitochondrial fractions were prepared from rat skeletal muscle without the use of proteolytic enzymes. The products of [U-14C]hexadecanoate oxidation by rat skeletal muscle mitochondrial fractions were analysed by h.p.l.c. with on-line radiochemical detection. In the presence of 1 mM-carnitine, 70% of the products is acetylcarnitine. In agreement with Veerkamp et al. [Veerkamp, van Moerkerk, Glatz, Zuurveld, Jacobs & Wagenmakers (1986) Biochem. Med. Metab. Biol. 35, 248-259] 14CO2 release is shown to be an unreliable estimate of flux through beta-oxidation in skeletal muscle mitochondrial fractions. The flux through beta-oxidation is recorded unambiguously polarographically in the presence of 1 mM-carnitine and the absence of citrate cycle intermediates.

MeSH Terms
Acetylcarnitine/metabolism Animals Carbon Radioisotopes Carnitine/pharmacology Chromatography, High Pressure Liquid Malates/pharmacology Male Mitochondria, Muscle/drug effects,metabolism Oxidation-Reduction Palmitic Acids/metabolism Palmitoylcarnitine/metabolism Rats Rats, Inbred Strains Tissue Distribution
Chemicals
Carbon Radioisotopes Malates Palmitic Acids Palmitoylcarnitine Acetylcarnitine malic acid Carnitine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Watmough N J
Department of Pharmacological Sciences, Medical School, University of Newcastle upon Tyne, U.K.
Bhuiyan A K
Bartlett K
Sherratt H S
Turnbull D M
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2530541
Published
1988-07-15
Pages
541-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1149331
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2530541 PMC Full Text View journal details
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