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PMID: 3174625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two cis-acting elements responsible for posttranscriptional trans-regulation of gene expression of human T-cell leukemia virus type I.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 19 ·1988-10-00 ·Pages 7124-8

Seiki M, Inoue J, Hidaka M, Yoshida M

Abstract

The pX sequence of human T-cell leukemia virus type I codes for two nuclear proteins, p40tax and p27rex, and a cytoplasmic protein, p21x-III.p40tax activates transcription from the long terminal repeat (LTR), whereas p27rex modulates posttranscriptional processing to accumulate gag and env mRNAs that retain intron sequences. In this paper, we identify two cis-acting sequence elements needed for regulation by p27rex: a 5' splice signal and a specific sequence in the 3' LTR. These two sequence elements are sufficient for regulation by p27rex; expression of a cellular gene (metallothionein I) became sensitive to rex regulation when the LTR was inserted at the 3' end of this gene. The requirement for these two elements suggests an unusual regulatory mechanism of RNA processing in the nucleus.

MeSH Terms
Gene Expression Regulation Human T-lymphotropic virus 1/genetics Humans Introns Plasmids Protein Processing, Post-Translational RNA Splicing Repetitive Sequences, Nucleic Acid Transfection
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Seiki M
Department of Viral Oncology, Cancer Institute, Tokyo, Japan.
Inoue J
Hidaka M
Yoshida M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.19.7124
Published
1988-10-00
Pages
7124-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282136
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.19.7124 PMC Full Text View journal details
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