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PMID: 3035544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional (p40x) and post-transcriptional (p27x-III) regulators are required for the expression and replication of human T-cell leukemia virus type I genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 11 ·1987-06-00 ·Pages 3653-7

Inoue J, Yoshida M, Seiki M

Abstract

The pX sequence of human T-cell leukemia virus type I codes for three products: p40x, p27x-III, and p21x-III. p40x is a transcriptional trans-activator that activates not only the viral long terminal repeat but also cellular genes for interleukin 2 and its receptor. p27x-III and p21x-III are not required for transcriptional activation, and their functions were unknown. Cotransfection experiments with defective human T-cell leukemia virus type I proviruses and various pX expression plasmids revealed that p27x-III, in addition to p40x, was required for gag gene expression. Furthermore, it was shown that p27x-III induced accumulation of a high level of unspliced viral gag mRNA. These results indicate that p27x-III is a post-transcriptional modulator of viral RNA whose transcription has been fully activated by p40x.

MeSH Terms
Cell Line DNA Replication DNA Restriction Enzymes Deltaretrovirus/genetics Exons Genes, Regulator Genes, Viral Humans Plasmids RNA Processing, Post-Transcriptional Transcription, Genetic Transfection Virus Replication
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Inoue J
Yoshida M
Seiki M
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.11.3653
Published
1987-06-00
Pages
3653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304933
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.11.3653 PMC Full Text View journal details
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