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PMID: 3132611 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA-binding factors of B lymphoid cells are susceptible to limited proteolytic cleavage during nuclear extract preparation.

Molecular and cellular biology ·Vol. 8 ·No. 4 ·1988-04-00 ·Pages 1812-5

Mather EL

Abstract

DNA-binding proteins that interact with the 3' end of the mouse mu immunoglobulin heavy chain gene were identified by the electrophoretic mobility shift assay. Complexes of distinctly different mobilities were formed by extracts prepared from B lymphoid lines representing different stages of maturation. The apparent stage-specific differences are shown to be due to proteolytic events that occurred during extract preparation.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Line Cell Nucleus/metabolism DNA-Binding Proteins/metabolism Genes, Immunoglobulin Immunoglobulin Heavy Chains/genetics Lymphoma/immunology Peptide Hydrolases
Chemicals
DNA-Binding Proteins Immunoglobulin Heavy Chains Peptide Hydrolases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Mather E L
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.
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26 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.4.1812-1815.1988
Published
1988-04-00
Pages
1812-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363344
Subset
IM
Grants
NIAID NIH HHS · AI20443 · United States
NIGMS NIH HHS · GM39228 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.4.1812-1815.1988 PMC Full Text View journal details
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