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PMID: 3131736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo functional analysis of in vitro protein binding sites in the immunoglobulin heavy chain enhancer.

Nucleic acids research ·Vol. 16 ·No. 8 ·1988-04-25 ·Pages 3239-53

Tsao BP, Wang XF, Peterson CL, Calame K

Abstract

We have systematically investigated the functional role of protein binding sites within the mouse immunoglobulin heavy chain enhancer which we previously identified by in vitro binding studies (1,2). Each binding site was deleted, mutant enhancers were cloned 3' of the chloramphenicol acetyl transferase gene in the vector pA10CAT2 and transfected into plasmacytoma cells. We demonstrate that the newly identified site E, located at 324-338 bp, is important for enhancer function; previously identified sites B(uE1), Cl(uE2), C2(uE3) and C3 were also shown to be important for enhancer activity. Sites A and D are not required for IgH enhancer function, as assayed by our methods. Thus, including the octamer site, six protein binding sites which bind at least six different proteins are important for enhancer function in vivo.

MeSH Terms
Animals Base Sequence Binding Sites DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Genes, Immunoglobulin Immunoglobulin Heavy Chains/genetics Mice Molecular Sequence Data Protein Binding
Chemicals
DNA-Binding Proteins Immunoglobulin Heavy Chains
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tsao B P
Department of Biological Chemistry, UCLA School of Medicine 90024.
Wang X F
Peterson C L
Calame K
References (42)
42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.8.3239
Published
1988-04-25
Pages
3239-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336491
Subset
IM
Grants
NIGMS NIH HHS · GM29361 · United States
External Links
PubMed source DOI: 10.1093/nar/16.8.3239 PMC Full Text View journal details
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