An ARS element from Drosophila melanogaster telomeres contains the yeast ARS core and bent replication enhancer.

Nucleic acids research ·Vol. 16 ·No. 3 ·1988-02-11 ·Pages 1169-80

Gragerov AI, Danilevskaya ON, Didichenko SA, Kaverina EN

Abstract

We have sequenced the 0.7-kb-long fragment of Drosophila DNA which ensures the autonomous replication of plasmids in yeast. Deletion mapping has shown the ARS element to consist of at least two domains: the core having the consensus 11-bp sequence TAAATATAAAT and the enhancer which is no more than 90 bp long and is located at the 3'-end of the A-rich core strand. Neither domain per se ensures plasmid replication in yeast. A comparison of the enhancer sequence with the sequences of 14 different ARS elements failed to reveal significant homology areas. Most probably the ARS flanks that are adjacent to the core and act as enhancer do not carry any consensus. They may determine a peculiar structural feature of DNA (for example bends) which are necessary for the protein-ARS interaction.

MeSH Terms

Animals Base Sequence DNA Replication Drosophila melanogaster/genetics Enhancer Elements, Genetic Molecular Sequence Data Nucleic Acid Conformation Replicon Saccharomyces cerevisiae/genetics

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gragerov A I

Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.

Danilevskaya O N

Didichenko S A

Kaverina E N

References (25)

25 references, click to expand

Sequence-induced DNA curvature at the bacteriophage lambda origin of replication.
Nature. 1985 Oct 3-9;317(6036):451-3 PMID: 2995831
Drosophila ARSs contain the yeast ARS consensus sequence and a replication enhancer.
Nucleic Acids Res. 1986 Aug 26;14(16):6633-48 PMID: 3092187
Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.
Mol Cell Biol. 1981 Jun;1(6):535-43 PMID: 6765606
Isolation of a yeast centromere and construction of functional small circular chromosomes.
Nature. 1980 Oct 9;287(5782):504-9 PMID: 6999364
Bent DNA at a yeast autonomously replicating sequence.
Nature. 1986 Nov 6-12;324(6092):87-9 PMID: 3785376
Deletion mutations affecting autonomously replicating sequence ARS1 of Saccharomyces cerevisiae.
Mol Cell Biol. 1984 Nov;4(11):2455-66 PMID: 6392851
Fine-structure analysis of the DNA sequence requirements for autonomous replication of Saccharomyces cerevisiae plasmids.
Mol Cell Biol. 1986 Jul;6(7):2354-63 PMID: 3023929
Evaluation of heterologous ARS activity in S. cerevisiae using cloned DNA from S. pombe.
Nucleic Acids Res. 1985 May 24;13(10):3711-22 PMID: 3892482
Localization and sequence analysis of yeast origins of DNA replication.
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1165-73 PMID: 6345070
Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
Gene. 1980 Jul;10(2):157-66 PMID: 6248420
Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae.
Nucleic Acids Res. 1984 Jan 25;12(2):1049-68 PMID: 6320114
Nucleotide sequence of the yeast plasmid.
Nature. 1980 Aug 28;286(5776):860-5 PMID: 6251374
DNA sequence analysis of ARS elements from chromosome III of Saccharomyces cerevisiae: identification of a new conserved sequence.
Nucleic Acids Res. 1986 Aug 11;14(15):6247-64 PMID: 3529036
An altered DNA conformation in origin region I is a determinant for the binding of SV40 large T antigen.
Cell. 1986 Mar 14;44(5):719-25 PMID: 3004744
Characterization of a yeast replication origin (ars2) and construction of stable minichromosomes containing cloned yeast centromere DNA (CEN3).
Gene. 1981 Nov;15(2-3):157-66 PMID: 7028571
The yeast ARS element, six years on: a progress report.
Yeast. 1985 Sep;1(1):1-14 PMID: 3916857
Pedigree analysis of plasmid segregation in yeast.
Cell. 1983 Oct;34(3):961-70 PMID: 6354471
DNA bending at adenine . thymine tracts.
Nature. 1986 Apr 10-16;320(6062):501-6 PMID: 3960133
Effect of ARS1 mutations on chromosome stability in Saccharomyces cerevisiae.
Mol Cell Biol. 1985 Jul;5(7):1676-84 PMID: 3894934
Structural requirements for the function of a yeast chromosomal replicator.
Cell. 1984 May;37(1):299-307 PMID: 6327056
Two modules from the hypersuppressive rho- mitochondrial DNA are required for plasmid replication in yeast.
Gene. 1984 Oct;30(1-3):47-61 PMID: 6392025
Eukaryotic DNA segments capable of autonomous replication in yeast.
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4559-63 PMID: 6449009
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Localization and sequence analysis of chloroplast DNA sequences of Chlamydomonas reinhardii that promote autonomous replication in yeast.
EMBO J. 1984 Feb;3(2):415-21 PMID: 16453506
Transformation of intact yeast cells treated with alkali cations.
J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.3.1169

Published

1988-02-11

Pages

1169-80

Language

English

Region

England

NLM ID

0411011

PMCID

PMC334745

Subset

Databases

GENBANK

X06883

External Links

PubMed source DOI: 10.1093/nar/16.3.1169 PMC Full Text View journal details

Analysis Services

An ARS element from Drosophila melanogaster telomeres contains the yeast ARS core and bent replication enhancer.

Contact

WeChat Official Account