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PMID: 3092187 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Drosophila ARSs contain the yeast ARS consensus sequence and a replication enhancer.

Nucleic acids research ·Vol. 14 ·No. 16 ·1986-08-26 ·Pages 6633-48

Mills JS, Kingsman AJ, Kingsman SM

Abstract

A number of restriction fragments that function as autonomously replicating sequences (ARSs) in yeast have been isolated from Drosophila melanogaster DNA. The behaviour in yeast of plasmids containing Drosophila ARS elements was studied and compared to that exhibited by the archetypal yeast ARS-1 plasmid. ARS functions were localised by subcloning and BAL-31 deletion analysis. These studies demonstrated the structural and functional complexity of Drosophila ARSs. Each Drosophila ARS element has at least two domains, one essential for replication (the replication sequence, RS) and a second (the replication enhancer, RE) which is essential for maximum function of the RS. The RS of three Drosophila ARSs was shown to contain a sequence identical to an 11 bp yeast ARS consensus sequence (5' A/T TTTATPuTTT A/T 3'). These observations lend support to the hypothesis that heterologous ARS elements may be of biological significance.

MeSH Terms
Animals Base Sequence DNA Replication DNA, Fungal/genetics Drosophila melanogaster/genetics Enhancer Elements, Genetic Escherichia coli/genetics Genes Genes, Fungal Genes, Regulator Genetic Vectors Plasmids Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mills J S
Kingsman A J
Kingsman S M
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.16.6633
Published
1986-08-26
Pages
6633-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311670
Subset
IM
Databases
GENBANK
X04285, X04286
External Links
PubMed source DOI: 10.1093/nar/14.16.6633 PMC Full Text View journal details
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