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PMID: 3121633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Urokinase-type plasminogen activator is induced in migrating capillary endothelial cells.

The Journal of cell biology ·Vol. 105 ·No. 6 Pt 1 ·1987-12-00 ·Pages 2535-41

Pepper MS, Vassalli JD, Montesano R, Orci L

Abstract

Cellular migration is an essential component of invasive biological processes, many of which have been correlated with an increase in plasminogen activator production. Endothelial cell migration occurs in vivo during repair of vascular lesions and angiogenesis, and can be induced in vitro by wounding a confluent monolayer of cells. By combining the wounded monolayer model with a substrate overlay technique, we show that cells migrating from the edges of an experimental wound display an increase in urokinase-type plasminogen activator (uPA) activity, and that this activity reverts to background levels upon cessation of movement, when the wound has closed. Our results demonstrate a direct temporal relationship between endothelial cell migration and uPA activity, and suggest that induction of uPA activity is a component of the migratory process.

MeSH Terms
Adrenal Cortex/blood supply Amiloride/pharmacology Animals Capillaries Cattle Cell Division/drug effects Cell Movement Cells, Cultured Endothelium, Vascular/cytology,drug effects,enzymology Enzyme Induction HeLa Cells/cytology,drug effects Humans Mitomycin Mitomycins/pharmacology Mitosis/drug effects Plasminogen/pharmacology Tetradecanoylphorbol Acetate/pharmacology Urokinase-Type Plasminogen Activator/biosynthesis
Chemicals
Mitomycins Mitomycin Amiloride Plasminogen Urokinase-Type Plasminogen Activator Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pepper M S
Institute of Histology and Embryology, University of Geneva Medical Center, Switzerland.
Vassalli J D
Montesano R
Orci L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.6.2535
Published
1987-12-00
Pages
2535-41
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114714
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.105.6.2535 PMC Full Text View journal details
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