Home LiteratureArticle Details
PMID: 3112525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new mutator strain of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 208 ·No. 1-2 ·1987-06-00 ·Pages 353-6

Viret JF, Alonso JC

Abstract

Bacillus subtilis strain SB1207, widely used in our laboratory, was found to be highly temperature-sensitive and to exhibit a strong SOS-independent mutator phenotype at elevated temperatures. Both chromosomal and plasmid-borne genes were affected by the mutator. Lethality and mutator phenotype could not be attributed to a replication shut off or to thymine starvation. Due to the high frequency of base misincorporation, the mutator phenotype probably results from an editing defect rather than from a post-replication defect (mismatch repair).

MeSH Terms
Bacillus subtilis/genetics Drug Resistance, Microbial Mutation Phenotype SOS Response, Genetics Streptomycin/pharmacology Temperature
Chemicals
Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Viret J F
Alonso J C
References (26)
26 references, click to expand
  1. Cloning and expression of the Escherichia coli recA gene in Bacillus subtilis.
    Gene. 1983 Nov;25(2-3):301-8 PMID: 6420239
  2. Physical properties of the DNA of bacteriophage SP50.
    Mol Gen Genet. 1967;100(1):39-55 PMID: 4967655
  3. Suppressor system in Bacillus subtilis 168.
    J Bacteriol. 1969 Mar;97(3):1397-402 PMID: 4975748
  4. Genetic effects of deoxyribonucleotide pool imbalances.
    Environ Mutagen. 1982;4(6):695-725 PMID: 6761109
  5. Mapping of the gene specifying DNA polymerase III of Bacillus subtilis.
    Mol Gen Genet. 1976 Mar 30;144(3):313-21 PMID: 818505
  6. Effects of a recA operator mutation on mutant phenotypes conferred by lexA and recF mutations.
    Mutat Res. 1982 Nov;106(1):11-26 PMID: 6219286
  7. Plasmid maintenance in Bacillus subtilis recombination-deficient mutants.
    Mol Gen Genet. 1987 Jun;208(1-2):349-52 PMID: 3112524
  8. The genetics and specificity of the constitutive excision repair system of Bacillus subtilis.
    Mol Gen Genet. 1983;190(3):481-6 PMID: 6410154
  9. Repair deficiency, mutator activity, and thermal prophage inducibility in dna-8132 strains of Bacillus subtilis.
    J Bacteriol. 1976 Jun;126(3):1037-41 PMID: 820681
  10. Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases.
    J Biol Chem. 1973 Nov 25;248(22):7688-700 PMID: 4201494
  11. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  12. Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis.
    Mol Gen Genet. 1970;108(3):277-87 PMID: 4990908
  13. Bacillus subtilis bacteriophage SPbeta: localization of the prophage attachment site, and specialized transduction.
    J Bacteriol. 1977 Jan;129(1):556-8 PMID: 401505
  14. Bacterial mutator genes and the control of spontaneous mutation.
    Annu Rev Genet. 1976;10:135-56 PMID: 797306
  15. Morphological and cell wall alterations in thermosensitive DNA mutants of Bacillus subtilis.
    Ann Inst Pasteur Microbiol (1985). 1985 Jan-Feb;136A(1):119-29 PMID: 3923900
  16. Genetic analysis of rec mutants of Bacillus subtilis. Evidence for at least six linkage groups.
    Mol Gen Genet. 1974 Mar 27;129(3):269-74 PMID: 4210037
  17. Asymmetric bidirectional replication of Bacillus subtilis chromosome.
    Nat New Biol. 1973 Aug 15;244(137):200-3 PMID: 4199318
  18. Temperature-sensitive mutants of B. subtilis defective in DNA synthesis.
    Cold Spring Harb Symp Quant Biol. 1968;33:307-12 PMID: 4977755
  19. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  20. Fidelity of DNA synthesis.
    Annu Rev Biochem. 1982;51:429-57 PMID: 6214209
  21. Spontaneous mutagenesis: the roles of DNA repair, replication, and recombination.
    Mutat Res. 1985 Jul;154(1):1-27 PMID: 3889622
  22. Revised genetic linkage map of Bacillus subtilis.
    Microbiol Rev. 1985 Jun;49(2):158-79 PMID: 2989674
  23. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168.
    Appl Environ Microbiol. 1977 Apr;33(4):989-93 PMID: 405929
  24. Properties of Bacillus subtilis 168 derivatives freed of their natural prophages.
    Gene. 1980 Dec;12(1-2):155-9 PMID: 6783474
  25. Mutations as possible replication errors in bacteria growing under conditions of thymine deficiency.
    Mutat Res. 1973 Sep;19(3):281-93 PMID: 4201996
  26. A gene controlling segregation of the Bacillus subtilis plasmid pC194.
    Mol Gen Genet. 1985;198(3):427-31 PMID: 3925293
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330465
Published
1987-06-00
Pages
353-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330465
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2