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PMID: 3108886 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Purified prion proteins and scrapie infectivity copartition into liposomes.

Gabizon R, McKinley MP, Prusiner SB

Abstract

Considerable evidence indicates that the scrapie prion protein (PrP 27-30) is required for infectivity. Aggregates of PrP 27-30 form insoluble amyloid rods that resist dissociation by nondenaturing detergents. Mixtures of the detergent cholate and phospholipids were found to solubilize purified PrP 27-30 in the form of detergent-lipid-protein complexes. Removal of the cholate by dialysis resulted in the formation of closed liposomes. Both the detergent-lipid-protein complexes and the liposomes often but not always exhibited a 10-fold increase in scrapie infectivity compared to that observed with the rods. No evidence for a prion-associated nucleic acid could be found when the phospholipid vesicles containing PrP 27-30 were digested with nucleases and Zn2+ under conditions that allowed hydrolysis of exogenously added nucleic acids. No filamentous or rod-shaped particles were found amongst prion liposomes by electron microscopy in our search for a putative filamentous "scrapie virus." The partitioning of PrP 27-30 and scrapie infectivity into phospholipid vesicles contends that PrP 27-30 has a central role in scrapie pathogenesis, establishes that the prion amyloid rods are not essential for infectivity, and argues that prions are fundamentally different from viruses.

MeSH Terms
Animals Cricetinae DNA/metabolism Light Liposomes Microscopy, Electron Nucleic Acid Hybridization Prions/analysis,pathogenicity,ultrastructure Scattering, Radiation Viral Proteins/genetics,isolation & purification
Chemicals
Liposomes Prions Viral Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gabizon R
McKinley M P
Prusiner S B
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1987-06-00
Pages
4017-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305012
Subset
IM
Grants
NIA NIH HHS · AG02132 · United States
NINDS NIH HHS · NS14069 · United States
NINDS NIH HHS · NS22786 · United States
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