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PMID: 2870469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conservation of the cellular gene encoding the scrapie prion protein.

Nucleic acids research ·Vol. 14 ·No. 5 ·1986-03-11 ·Pages 2035-44

Westaway D, Prusiner SB

Abstract

The major protein, PrP 27-30, in purified preparations of hamster scrapie prions is encoded within the genome of the experimental host. DNA sequences related to a PrP cDNA clone can be detected in a wide variety of organisms under relatively stringent conditions where the only signal generated by hamster or mouse DNA corresponds to the PrP gene. Three hosts for scrapie, goat, sheep and rat gave strong hybridization signals. In addition, three invertebrate DNAs reacted with the PrP probe, in the order nematode-Drosophila much greater than yeast. Thus, the sequences detected in goat, sheep, rat, nematode, Drosophila and possibly yeast DNA may arise from authentic PrP genes. This evolutionary conservation is consistent with the notion that PrP proteins participate in essential cellular processes.

MeSH Terms
Animals Cloning, Molecular Cricetinae DNA/genetics Genes Mice Nucleic Acid Hybridization PrP 27-30 Protein Prions Sequence Homology, Nucleic Acid Viral Proteins/genetics
Chemicals
Prions Viral Proteins PrP 27-30 Protein DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Westaway D
Prusiner S B
References (23)
23 references, click to expand
  1. Mapping of behaviour in Drosophila mosaics.
    Nature. 1972 Dec 29;240(5383):527-35 PMID: 4568399
  2. Prions: novel infectious pathogens.
    Adv Virus Res. 1984;29:1-56 PMID: 6241423
  3. Genetic control of scrapie and Creutzfeldt-Jakob disease in mice.
    J Immunol. 1983 Jul;131(1):491-6 PMID: 6408182
  4. Creutzfeldt-Jakob disease prion proteins in human brains.
    N Engl J Med. 1985 Jan 10;312(2):73-8 PMID: 3917302
  5. The Drosophila ras oncogenes: structure and nucleotide sequence.
    Cell. 1984 Jul;37(3):1027-33 PMID: 6430564
  6. Purification and structural studies of a major scrapie prion protein.
    Cell. 1984 Aug;38(1):127-34 PMID: 6432339
  7. Unusual resistance to ionizing radiation of the viruses of kuru, Creutzfeldt-Jakob disease, and scrapie.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6268-70 PMID: 104301
  8. The Drosophila EGF receptor gene homolog: conservation of both hormone binding and kinase domains.
    Cell. 1985 Mar;40(3):599-607 PMID: 2982499
  9. Isolation of the proto-oncogene c-myb from D. melanogaster.
    Cell. 1985 Jun;41(2):449-56 PMID: 3921261
  10. Experimental production of scrapie in goats.
    J Comp Pathol. 1959 Jul;69:300-12 PMID: 14430941
  11. From the gene to behavior.
    JAMA. 1971 Nov 15;218(7):1015-22 PMID: 4942064
  12. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
    Cell. 1984 Mar;36(3):607-12 PMID: 6365329
  13. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain.
    Nature. 1985 May 23-29;315(6017):331-3 PMID: 3923361
  16. The DNA of Caenorhabditis elegans.
    Genetics. 1974 May;77(1):95-104 PMID: 4858229
  17. The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.
    Nucleic Acids Res. 1984 Jul 25;12(14):5627-38 PMID: 6087294
  18. Creutzfeldt-Jakob disease virus isolations from the Gerstmann-Sträussler syndrome with an analysis of the various forms of amyloid plaque deposition in the virus-induced spongiform encephalopathies.
    Brain. 1981 Sep;104(3):559-88 PMID: 6791762
  19. Unconventional viruses and the origin and disappearance of kuru.
    Science. 1977 Sep 2;197(4307):943-60 PMID: 142303
  20. HOSPITAL SERVICES FOR THE SUBNORMAL.
    Lancet. 1963 Nov 30;2(7318):1165-6 PMID: 14072926
  21. EXPERIMENTAL TRANSMISSION OF MOUSE PASSAGED SCRAPIE TO GOATS, SHEEP, RATS AND HAMSTERS.
    J Comp Pathol. 1965 Apr;75:147-57 PMID: 14319384
  22. A protease-resistant protein is a structural component of the scrapie prion.
    Cell. 1983 Nov;35(1):57-62 PMID: 6414721
  23. A cellular gene encodes scrapie PrP 27-30 protein.
    Cell. 1985 Apr;40(4):735-46 PMID: 2859120
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.5.2035
Published
1986-03-11
Pages
2035-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339641
Subset
IM
Grants
NIA NIH HHS · AG02132 · United States
NINDS NIH HHS · NS14069 · United States
NINDS NIH HHS · NS22786 · United States
External Links
PubMed source DOI: 10.1093/nar/14.5.2035 PMC Full Text View journal details
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