Home LiteratureArticle Details

PMID: 3088543 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vitro transcription of bacteriophage phi 29 DNA. Correlation between in vitro and in vivo promoters.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4731-41

Mellado RP, Barthelemy I, Salas M

Abstract

The phi 29 DNA in vitro transcription initiation sites have been accurately mapped by S1 protection experiments. The results obtained indicated that the B. subtilis RNA polymerase containing the sigma 43 subunit basically recognized the same set of phi 29 promoters in vitro as those used in vivo. In addition, the sequence of the phi 29 early A2a promoter used both in vitro and in vivo has been determined as well as the precise nucleotide where initiation of transcription from the C2 promoter occurs in vitro.

MeSH Terms

Bacillus subtilis/genetics Bacteriophages/genetics Chromosome Mapping DNA, Viral/genetics DNA-Directed RNA Polymerases/metabolism Promoter Regions, Genetic RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Sequence Homology, Nucleic Acid Transcription, Genetic

Chemicals

DNA, Viral RNA, Messenger RNA, Viral DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Mellado R P

Barthelemy I

Salas M

References (12)

12 references, click to expand

Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections.
J Virol. 1976 Aug;19(2):495-500 PMID: 822174
Mapping adenines, guanines, and pyrimidines in RNA.
Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
Purification of Bacillus subtilis RNA polymerase with heparin-agarose. In vitro transcription of phi 29 DNA.
J Biol Chem. 1979 Sep 25;254(18):9220-6 PMID: 113409
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Nucleotide sequence and transcription of a bacteriophage 29 early promoter.
J Biol Chem. 1985 May 25;260(10):5950-5 PMID: 3997806
Transcription of sea urchin histone genes in Escherichia coli.
Nucleic Acids Res. 1981 Aug 25;9(16):3889-906 PMID: 6458017
Nucleotide sequences of transcription and translation initiation regions in Bacillus phage phi 29 early genes.
J Biol Chem. 1982 Jan 25;257(2):1053-62 PMID: 6274853
Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29.
Nucleic Acids Res. 1982 Oct 11;10(19):5785-98 PMID: 6292852
A new mechanism for the initiation of replication of phi 29 and adenovirus DNA: priming by the terminal protein.
Curr Top Microbiol Immunol. 1984;109:89-106 PMID: 6421547
In vitro transcription of the Bacillus subtilis phage phi 29 DNA by Bacillus subtilis and Escherichia coli RNA polymerases.
Nucleic Acids Res. 1984 Feb 24;12(4):1943-60 PMID: 6322128
Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1446-50 PMID: 6262800

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.12.4731

Published

1986-06-25

Pages

4731-41

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311487

Subset

Grants

NIGMS NIH HHS · 5 R01 GM27242-06 · United States

External Links

PubMed source DOI: 10.1093/nar/14.12.4731 PMC Full Text View journal details

Analysis Services

In vitro transcription of bacteriophage phi 29 DNA. Correlation between in vitro and in vivo promoters.

Contact

WeChat Official Account