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PMID: 3080747 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 3 ·1986-02-00 ·Pages 701-5

Gunaratne PH, Mansukhani A, Lipari SE, Liou HC, Martindale DW, Goldberg ML

Abstract

Approximately 170 kilobase pairs (kb) of contiguous DNA sequences derived from bands 3A3,4 of the Drosophila melanogaster X chromosome have been isolated by molecular cloning. Sequences required for the wild-type expression of the zeste locus are located within a 6-kb fragment of this chromosomal region, as shown by phenotypic rescue of zeste mutants in P element-mediated germ-line transformation. Expression of zeste is correlated with a 2.2-kb poly(A)+ RNA species transcribed at all postzygotic stages of Drosophila development. Many zeste alleles, including several producing neomorphic phenotypes, are not associated with detectable rearrangements of DNA.

MeSH Terms
Animals Base Sequence Chromosome Aberrations Cloning, Molecular DNA/isolation & purification Drosophila melanogaster/anatomy & histology,genetics Female Male Phenotype Transcription, Genetic Transformation, Genetic X Chromosome
Chemicals
DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gunaratne P H
Mansukhani A
Lipari S E
Liou H C
Martindale D W
Goldberg M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.3.701
Published
1986-02-00
Pages
701-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322932
Subset
IM
Grants
NIGMS NIH HHS · GM31935 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.3.701 PMC Full Text View journal details
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