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PMID: 16453549 Published · ppublish English Journal Article

Functional analysis of the white gene of Drosophila by P-factor-mediated transformation.

The EMBO journal ·Vol. 3 ·No. 9 ·1984-09-00 ·Pages 2077-85

Gehring WJ, Klemenz R, Weber U, Kloter U

Abstract

A 12-kb DNA segment spanning the white (w) locus of Drosophila has been inserted into a P-transposon vector and used for P-factor-mediated germ-line transformation. Several red-eyed transformants were recovered which complement the white mutant phenotype. Analysis of the eye pigments and the interaction with the zeste mutation indicates that the w gene inserted at several new chromosomal sites is expressed normally. The tissue-specific accumulation of w transcripts, as studied by in situ hybridization to tissue sections, is the same in transformant and wild-type larvae. This indicates that all the genetic information specified by the w locus is contained within this 12-kb segment of DNA. By secondary mobilization it was shown that the w sequences have been inserted as a functional P(w) transposon which is capable of further transposition.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gehring W J
Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Klemenz R
Weber U
Kloter U
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-09-00
Pages
2077-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557646
External Links
PubMed source PMC Full Text View journal details
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