Home LiteratureArticle Details
PMID: 3067082 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination.

Molecular & general genetics : MGG ·Vol. 214 ·No. 1 ·1988-09-00 ·Pages 80-4

Stirling CJ, Stewart G, Sherratt DJ

Abstract

The heritable stability of the multicopy plasmid ColE1 and its natural relatives, requires the presence in the plasmid of a site (cer in ColE1) that acts as a substrate for site-specific recombination, thereby maintaining plasmids in the monomeric state. Multimerization, promoted by homologous recombination, leads to plasmid loss. Here we show that the Escherichia coli chromosome encodes at least two unlinked functions that act on cer and its analogous sites, to promote stabilizing site-specific recombination. One of these functions is encoded by a gene residing on a cosmid that also contains the argI and pyrB genes, mapping it to the 96-97 min region of the E. coli map.

MeSH Terms
Bacterial Proteins/genetics Colicins/genetics Cosmids Escherichia coli/genetics Genes, Bacterial Genetic Complementation Test Mutation Phenotype Plasmids Recombination, Genetic Restriction Mapping
Chemicals
Bacterial Proteins Colicins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stirling C J
Institute of Genetics, University of Glasgow, UK.
Stewart G
Sherratt D J
References (21)
21 references, click to expand
  1. Site-specific recombination promotes plasmid amplification in yeast.
    Cell. 1986 Aug 15;46(4):541-50 PMID: 3524855
  2. The complete nucleotide sequence of the colicinogenic plasmid ColA. High extent of homology with ColE1.
    Mol Gen Genet. 1988 Feb;211(2):231-43 PMID: 2832701
  3. D protein of miniF plasmid acts as a repressor of transcription and as a site-specific resolvase.
    Nucleic Acids Res. 1986 Dec 22;14(24):9713-28 PMID: 3027661
  4. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.
    Gene. 1984 Feb;27(2):131-49 PMID: 6327463
  5. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  6. A site-specific recombination function in Staphylococcus aureus plasmids.
    J Bacteriol. 1987 Jun;169(6):2601-10 PMID: 3584064
  7. Multimer resolution systems of ColE1 and ColK: localisation of the crossover site.
    Mol Gen Genet. 1985;201(2):334-8 PMID: 3003536
  8. Site-specific recombinases: changing partners and doing the twist.
    J Bacteriol. 1986 Feb;165(2):341-7 PMID: 3003022
  9. Location of the site-specific recombination system of R46: a function necessary for plasmid maintenance.
    J Gen Microbiol. 1986 Apr;132(4):1009-20 PMID: 3020154
  10. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  11. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.
    Cell. 1984 Apr;36(4):1097-103 PMID: 6323019
  12. Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.
    Cell. 1984 Jan;36(1):203-9 PMID: 6319008
  13. Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.
    J Biol Chem. 1981 Mar 10;256(5):2143-53 PMID: 6257703
  14. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  15. Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site.
    EMBO J. 1988 Mar;7(3):851-8 PMID: 3294000
  16. Rapid identification of Escherichia coli transformed by pBR322 carrying inserts at the PstI Site.
    Anal Biochem. 1982 May 1;122(1):85-8 PMID: 6285764
  17. Cellular factors couple recombination with growth phase: characterization of a new component in the lambda site-specific recombination pathway.
    Cell. 1987 Sep 11;50(6):901-8 PMID: 2957063
  18. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  19. Nucleotide sequence of the partition function of Escherichia coli plasmid ColE1.
    DNA. 1985 Oct;4(5):351-5 PMID: 3908032
  20. A novel role for site-specific recombination in maintenance of bacterial replicons.
    Cell. 1981 Sep;25(3):729-36 PMID: 7026049
  21. Site-specific recombination in transposition and plasmid stability.
    Cold Spring Harb Symp Quant Biol. 1984;49:227-33 PMID: 6099237
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00340183
Published
1988-09-00
Pages
80-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00340183
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2