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PMID: 2832701 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The complete nucleotide sequence of the colicinogenic plasmid ColA. High extent of homology with ColE1.

Molecular & general genetics : MGG ·Vol. 211 ·No. 2 ·1988-02-00 ·Pages 231-43

Morlon J, Chartier M, Bidaud M, Lazdunski C

Abstract

The complete nucleotide sequence of the colicinogenic plasmid ColA has been determined. The plasmid DNA consists of 6720 bp (molecular weight 4.48 X 10(6]. Fifteen putative biological functions have been identified using the functional map previously determined. These include 11 genes and 3 DNA sites. Nine genes encode proteins of which 3 have been fully characterized. The replication region of ColA coding for RNAI and RNAII is highly homologous to that of ColE1 and Clo DF13. The same holds true for the site-specific recombination region containing palindromic symmetry and involved in stable maintenance of the plasmids. A high percentage of homology has been detected for putative mobility proteins encoded by ColA and ColE1. The exclusion proteins are also highly homologous.

MeSH Terms
Amino Acid Sequence Base Sequence Colicins/genetics DNA Restriction Enzymes Escherichia coli/genetics Molecular Sequence Data Nucleic Acid Conformation Plasmids Sequence Homology, Nucleic Acid
Chemicals
Colicins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morlon J
Centre de Biochimie et de Biologie Moléculaire du CNRS, Marseille, France.
Chartier M
Bidaud M
Lazdunski C
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330599
Published
1988-02-00
Pages
231-43
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330599
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