Origin and direction of replication of the bacteriocinogenic plasmid Clo DF13.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 71-80

Stuitje AR, Veltkamp E, Weijers PJ, Nijkamp HJ

Abstract

Cairn's type replicative intermediates of both the wildtype Clo DF13 plasmid and the copy mutant CLO DF13 cop3 were isolated by dye-buoyant density centrifugation. Replicative intermediates were linearized at the HpaI or Sa1I cleavage site, and examined with the electron-microscope. The data show that replication of both the Clo DF13 wild type plasmid and the Clo DF13 cop3 plasmid, initiates at about 2.8% on the physical map. Replication proceeds unindirectionally and counterclockwise on this map.

MeSH Terms

Bacteriocins/biosynthesis DNA Replication DNA Restriction Enzymes DNA, Bacterial/metabolism Escherichia coli/metabolism Microscopy, Electron Nucleic Acid Conformation Plasmids

Chemicals

Bacteriocins DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Stuitje A R

Veltkamp E

Weijers P J

Nijkamp H J

References (14)

14 references, click to expand

Origin of replication of colicin E1 plasmid DNA.
Proc Natl Acad Sci U S A. 1977 May;74(5):1865-9 PMID: 325558
In vitro construction of deletion mutants of the bacteriocinogenic plasmid Clo DF13.
Nucleic Acids Res. 1978 Jun;5(6):1801-20 PMID: 353730
Isolation and characterization of a Clo DF13::Tn901 plasmid mutant with thermosensitive control of DNA replication.
J Bacteriol. 1978 Aug;135(2):612-21 PMID: 355238
Integration of a transposable DNA sequence which mediates ampicillin resistance into Clo DF13 plasmid DNA: determination of the site and orientation of TnA insertions.
Plasmid. 1978 Feb;1(2):204-17 PMID: 372963
Characterization of a replication mutant of the bacteriocinogenic plasmid Clo DF13.
Biochim Biophys Acta. 1976 Mar 17;425(3):356-67 PMID: 769825
Binding of bacteriocin Clo DF13 to Clo DF13 plasmid deoxyribonucleic acid in vivo and in vitro.
Antimicrob Agents Chemother. 1975 Sep;8(3):356-65 PMID: 1101824
Two replication initiation sites on R-plasmid DNA.
Mol Gen Genet. 1975 Sep 15;140(1):39-50 PMID: 1102950
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
Proteins synthesized by a non-induced bacteriocinogenic factor in minicells of Escherichia coli.
Mol Gen Genet. 1972;115(4):314-23 PMID: 4338179
Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256-9 PMID: 4601583
Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4 PMID: 4601821
Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication.
J Bacteriol. 1974 Dec;120(3):1227-37 PMID: 4612010
DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1.
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538-44 PMID: 4922634
MINIATURE escherichia coli CELLS DEFICIENT IN DNA.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):321-6 PMID: 16591472

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.1.71

Published

1979-01-00

Pages

71-80

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327674

Subset

External Links

PubMed source DOI: 10.1093/nar/6.1.71 PMC Full Text View journal details

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Origin and direction of replication of the bacteriocinogenic plasmid Clo DF13.

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