Home LiteratureArticle Details
PMID: 3012271 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A molecular genetic approach to the functioning of the immunity protein to colicin A.

Molecular & general genetics : MGG ·Vol. 202 ·No. 3 ·1986-03-00 ·Pages 455-60

Geli V, Baty D, Crozel V, Morlon J, Lloubes R, Pattus F, Lazdunski C

Abstract

A plasmid (pColAF1), derived from pColA, and lacking the region encoding Cai (colicin A immunity protein) and Cal (colicin A lysis protein) has been constructed. The strains carrying pColAF1 produce normal amounts of colicin A which remains in the cell cytoplasm and does not result in loss of viability. Similar results have also been obtained for transposon insertion mutants lacking Cai. Structure prediction analysis indicates that four peptide regions of Cai might span the cytoplasmic membrane. Since the NH2- and COOH-terminal regions are charged, this analysis suggests a topology of the 178 residues polypeptide chain in which regions 38 to 70 and 124 to 143 might be exposed at the outer side of the cytoplasmic membrane. With mutants constructed using recombinant DNA techniques, we could demonstrate that the removal of a 30 residue COOH-terminal region, and mutations altering the surface exposed loop comprised of aminoacid residues 124-143 abolish the protecting function of Cai.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Colicins/antagonists & inhibitors DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Mutation Plasmids Protein Conformation
Chemicals
Bacterial Proteins Colicins DNA Transposable Elements colicin immunity proteins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Geli V
Baty D
Crozel V
Morlon J
Lloubes R
Pattus F
Lazdunski C
References (38)
38 references, click to expand
  1. Biosynthesis and export of colicin A in Citrobacter freundii CA31.
    Eur J Biochem. 1981 Jun 1;116(3):615-20 PMID: 6455292
  2. Colicin E3 and its immunity genes.
    J Mol Biol. 1985 Mar 20;182(2):217-27 PMID: 3889348
  3. Organization of the colicin Ib gene. Promoter structure and immunity domain.
    J Biol Chem. 1984 Jul 25;259(14):8764-8 PMID: 6204975
  4. Colicin K acts by forming voltage-dependent channels in phospholipid bilayer membranes.
    Nature. 1978 Nov 9;276(5684):159-63 PMID: 740032
  5. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  6. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  7. E3 immunity substance. A protein from e3-colicinogenic cells that accounts for their immunity to colicin E3.
    J Biol Chem. 1974 Jan 25;249(2):445-53 PMID: 4588567
  8. Physical map of pColA-CA31, an amplifiable plasmid, and location of colicin A structural gene.
    Gene. 1982 Mar;17(3):317-21 PMID: 7049840
  9. Nucleotide sequence of the gene for the immunity protein to colicin A. Analysis of codon usage of immunity proteins as compared to colicins.
    Eur J Biochem. 1984 Oct 1;144(1):73-8 PMID: 6383827
  10. Studies of colicin induction with an imm- col+ mutant of the plasmid colicin E1.
    Mol Gen Genet. 1979 Jun 20;173(3):333-8 PMID: 384154
  11. Colicin E2 is DNA endonuclease.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3989-93 PMID: 1069283
  12. Mode of action of colicins Ia, E1 and K.
    Zentralbl Bakteriol Orig A. 1979 Jun;244(1):105-20 PMID: 388932
  13. Colicinogenic mutant of ColE1 plasmid that fails to confer immunity to colicin E1.
    Gene. 1978 Feb;3(1):1-7 PMID: 344141
  14. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  15. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  16. Purification and properties of colicin E3 immunity protein.
    J Biol Chem. 1974 Jan 25;249(2):438-44 PMID: 4588566
  17. Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression.
    Nucleic Acids Res. 1984 Sep 11;12(17):6727-39 PMID: 6091036
  18. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  19. Purification and molecular properties of a new colicin.
    Eur J Biochem. 1979 Jun 1;96(3):519-24 PMID: 380984
  20. Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31.
    Mol Gen Genet. 1983;192(3):500-5 PMID: 6318041
  21. Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):579-83 PMID: 6403939
  22. Complete nucleotide sequence of the structural gene for colicin A, a gene translated at non-uniform rate.
    J Mol Biol. 1983 Oct 25;170(2):271-85 PMID: 6313941
  23. Molecular characterisation of the colicin E2 operon and identification of its products.
    Mol Gen Genet. 1985;198(3):465-72 PMID: 3892228
  24. Purification and characterization of a complex between cloacin and its immunity protein isolated from Enterobacter cloacae (Clo DF13). Dissociation and reconstitution of the complex.
    Eur J Biochem. 1977 Feb 15;73(1):107-14 PMID: 402267
  25. Studies on the mechanism of action of channel-forming colicins using artificial membranes.
    J Membr Biol. 1984;79(2):105-18 PMID: 6086931
  26. cea-kil operon of the ColE1 plasmid.
    J Bacteriol. 1983 Mar;153(3):1479-85 PMID: 6298187
  27. Molecular structure of the immunity gene and immunity protein of the bacteriocinogenic plasmid Clo DF13.
    Nucleic Acids Res. 1980 Oct 10;8(19):4349-63 PMID: 6253914
  28. Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin.
    Nucleic Acids Res. 1983 Jun 11;11(11):3547-57 PMID: 6344012
  29. Deletions affecting the transposition of an antibiotic resistance gene.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):702-6 PMID: 322140
  30. The ins and outs of colicins. Part II. Lethal action, immunity and ecological implications.
    Microbiol Sci. 1984 Nov;1(8):203-5 PMID: 6444126
  31. Isolation, molecular and functional properties of the C-terminal domain of colicin A.
    EMBO J. 1983;2(9):1501-7 PMID: 11892802
  32. Prediction of the secondary structure of proteins from their amino acid sequence.
    Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 PMID: 364941
  33. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  34. Plasmid-determined immunity of Escherichia coli K-12 to colicin Ia Is mediated by a plasmid-encoded membrane protein.
    J Bacteriol. 1981 Dec;148(3):817-28 PMID: 6273383
  35. Lysis protein encoded by plasmid ColA-CA31. Gene sequence and export.
    Mol Gen Genet. 1985;199(1):95-100 PMID: 3889552
  36. Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae.
    J Gen Microbiol. 1972 Sep;72(2):349-55 PMID: 4562309
  37. Colicin A receptor: role of two Escherichia coli outer membrane proteins (OmpF protein and btuB gene product) and lipopolysaccharide.
    J Bacteriol. 1982 Aug;151(2):983-8 PMID: 7047502
  38. Studies on the depolarization of the Escherichia coli cell membrane by colicin E1.
    J Biol Chem. 1977 Aug 10;252(15):5491-7 PMID: 18466
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333276
Published
1986-03-00
Pages
455-60
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333276
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2