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PMID: 6344012 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin.

Nucleic acids research ·Vol. 11 ·No. 11 ·1983-06-11 ·Pages 3547-57

Mock M, Miyada CG, Gunsalus RP

Abstract

We have determined the nucleotide sequence of a segment of Co1E3 DNA coding for the carboxyl-terminal, catalytic peptide of colicin E3 and for the immunity protein. The end of the colicin E3 gene is separated from the beginning of the immunity gene by a nine-basepair intercistronic region, suggesting the two genes are expressed as a single transcriptional unit. The immunity gene is expressed, however, in E. coli strains containing Co1E3-pBR322 hybrid plasmids deleted for the 5'-end of the colicin gene. The DNA sequence also contains an unexpected open reading frame (ORF). This ORF is contained within the colicin gene and is in the +1 reading frame with respect to that gene. Plasmids containing the ORF, directed the synthesis of an 11 kilodalton protein in a cell-free, transcription-translation system.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Composition Base Sequence Colicins/genetics DNA, Bacterial/genetics Escherichia coli/genetics Escherichia coli Proteins Genes Genes, Bacterial Nucleic Acid Hybridization Plasmids Protein Biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins Colicins DNA, Bacterial Escherichia coli Proteins ceiC protein, E coli colicin immunity proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mock M
Miyada C G
Gunsalus R P
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.11.3547
Published
1983-06-11
Pages
3547-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325985
Subset
IM
Grants
NIAID NIH HHS · AI-07877 · United States
NIGMS NIH HHS · GM 29456 · United States
NIGMS NIH HHS · GM 30896 · United States
Databases
GENBANK
J01574, J01575, M14038
External Links
PubMed source DOI: 10.1093/nar/11.11.3547 PMC Full Text View journal details
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