Home LiteratureArticle Details
PMID: 3584064 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A site-specific recombination function in Staphylococcus aureus plasmids.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2601-10

Gennaro ML, Kornblum J, Novick RP

Abstract

All known small staphylococcal plasmids possess one or two recombination sites at which site-specific cointegrate formation occurs. One of these sites, RSA, is present on two small multicopy plasmids, pT181 and pE194; it consists of 24 base pairs of identity in the two plasmids, the "core," flanked by some 50 base pairs of decreasing homology. Here we show that recombination at RSA is recA independent and is mediated by a plasmid-encoded, trans-acting protein, Pre (plasmid recombination). Pre-mediated recombination is site specific in that it occurs within the core sequence of RSA in a recA1 host. Recombination also occurs between two intramolecular RSA sites. Unlike site-specific recombination systems encoded by other plasmids, Pre-RSA is not involved in plasmid maintenance.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Genes Genes, Bacterial Plasmids Promoter Regions, Genetic Recombination, Genetic Staphylococcus aureus/genetics
Chemicals
Bacterial Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gennaro M L
Kornblum J
Novick R P
References (41)
41 references, click to expand
  1. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.
    Virology. 1967 Sep;33(1):155-66 PMID: 4227577
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  4. Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus.
    Genetics. 1974 Apr;76(4):681-702 PMID: 4275652
  5. Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid.
    Mol Gen Genet. 1974;135(2):149-61 PMID: 4457755
  6. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids.
    J Bacteriol. 1975 Nov;124(2):597-601 PMID: 1184573
  7. Three distinct plasmids originating in the same Staphylococcus aureus strain.
    Arch Roum Pathol Exp Microbiol. 1976 Jan-Jun;35(1-2):111-8 PMID: 1008685
  8. Recombinational switch for gene expression.
    Science. 1977 Apr 8;196(4286):170-2 PMID: 322276
  9. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  10. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  11. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  12. Relationships between autonomous and integrated forms of tetracycline resistance plasmid in Staphylococcus aureus.
    Plasmid. 1979 Apr;2(2):216-24 PMID: 451050
  13. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps.
    Plasmid. 1979 Jan;2(1):109-29 PMID: 314115
  14. Site-specific recombination in phage mu.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1159-67 PMID: 158470
  15. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  16. Dissection of the transposition process: a transposon-encoded site-specific recombination system.
    Mol Gen Genet. 1979 Oct 1;175(3):267-74 PMID: 392228
  17. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  18. Organization of the pE194 genome.
    Mol Gen Genet. 1980;179(2):241-52 PMID: 6258012
  19. Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3428-32 PMID: 6267590
  20. A novel role for site-specific recombination in maintenance of bacterial replicons.
    Cell. 1981 Sep;25(3):729-36 PMID: 7026049
  21. Site-specific recombination and its role in the life cycle of bacteriophage P1.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:297-309 PMID: 6457723
  22. Transduction-related cointegrate formation between Staphylococcal plasmids: a new type of site-specific recombination.
    Plasmid. 1981 Sep;6(2):159-72 PMID: 6458059
  23. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies.
    J Bacteriol. 1982 May;150(2):804-14 PMID: 6279574
  24. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  25. Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):277-81 PMID: 6281761
  26. Closely related plasmids from Staphylococcus aureus and soil bacilli.
    Plasmid. 1982 Mar;7(2):152-62 PMID: 7079389
  27. Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4108-12 PMID: 6287465
  28. High-frequency transduction of pBR322 by cytosine-substituted T4 bacteriophage: evidence for encapsulation and transfer of head-to-tail plasmid concatemers.
    Plasmid. 1982 Jul;8(1):29-35 PMID: 6755510
  29. Determination of plasmid copy number by fluorescence densitometry.
    Plasmid. 1983 Mar;9(2):182-90 PMID: 6344110
  30. Simple and rapid method for disruption of bacteria for protein studies.
    Appl Environ Microbiol. 1983 Oct;46(4):941-3 PMID: 6639038
  31. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus.
    Plasmid. 1983 Nov;10(3):251-9 PMID: 6657777
  32. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.
    Cell. 1984 Apr;36(4):1097-103 PMID: 6323019
  33. Genetic switches by DNA inversions in prokaryotes.
    Biochim Biophys Acta. 1984 Jun 16;782(2):111-9 PMID: 6326835
  34. A site-specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross-over sites cix for the inversion of the C segment.
    EMBO J. 1982;1(11):1445-53 PMID: 6327269
  35. Staphylococcal plasmid cointegrates are formed by host- and phage-mediated general rec systems that act on short regions of homology.
    Mol Gen Genet. 1984;195(1-2):374-7 PMID: 6092862
  36. Control of pT181 replication I. The pT181 copy control function acts by inhibiting the synthesis of a replication protein.
    EMBO J. 1984 Oct;3(10):2399-405 PMID: 6499834
  37. Control of pT181 replication II. Mutational analysis.
    EMBO J. 1984 Oct;3(10):2407-14 PMID: 6437809
  38. Analysis of the requirements for transcription pausing in the tryptophan operon.
    J Mol Biol. 1985 Apr 5;182(3):397-409 PMID: 2409288
  39. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  40. Correction. A revision of the nucleotide sequence and functional map of pUB110.
    Plasmid. 1987 Jan;17(1):83-5 PMID: 3033723
  41. Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.
    Cell. 1984 Jan;36(1):203-9 PMID: 6319008
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2601-2610.1987
Published
1987-06-00
Pages
2601-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212133
Subset
IM
Grants
NIGMS NIH HHS · R01-GM 14372 · United States
Databases
GENBANK
J01764, J01765
External Links
PubMed source DOI: 10.1128/jb.169.6.2601-2610.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2