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PMID: 6327269 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A site-specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross-over sites cix for the inversion of the C segment.

The EMBO journal ·Vol. 1 ·No. 11 ·1982-00-00 ·Pages 1445-53

Iida S, Meyer J, Kennedy KE, Arber W

Abstract

The bacteriophage P1 genome carries an invertible C segment consisting of 3-kb unique sequences flanked by 0.6-kb inverted repeats. With insertion and deletion mutants of P1 derivatives the site-specific recombinase gene cin for C inversion) has been mapped adjacent to the C segment and the cix sites (for C inversion cross-over) have been located at the outside ends of the inverted repeats. Inversion of the C segment functions as a biological switch and controls expression of the gene(s) responsible for phage infectivity carried on the C segment. The cin gene product can promote recombination between a 'quasi- cix ' site on plasmid pBR322 and a cix site on P1 DNA. The junctions formed on the resulting co-integrate can also serve as cix sites. This observation implies a potential evolutionary process to bring genes under the control of a biological switch acting by DNA inversion.

MeSH Terms
Base Sequence Chromosome Deletion Coliphages/genetics Crossing Over, Genetic DNA Nucleotidyltransferases/genetics DNA Restriction Enzymes DNA Transposable Elements DNA, Recombinant/analysis Escherichia coli/genetics Genes Genes, Viral Plasmids
Chemicals
DNA Transposable Elements DNA, Recombinant DNA Nucleotidyltransferases cin recombinase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Iida S
Meyer J
Kennedy K E
Arber W
References (44)
44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1445-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553230
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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