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PMID: 3060856 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast.

Nucleic acids research ·Vol. 16 ·No. 23 ·1988-12-09 ·Pages 11091-105

Guzmán P, Ecker JR

Abstract

Procedures for the preparation, analysis and cloning of large DNA molecules from two different plant species are described. Arabidopsis and carrot protoplasts were used for the preparation of large DNA molecules in agarose "plugs" or in solution. Pulsed-field gel electrophoresis (PFGE) analysis of large plant DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA was at least 12 Mb. Large DNA preparations were shown to be useful for restriction enzyme analysis of the Arabidopsis genome using both frequent and infrequent cutting enzymes and for the molecular cloning of large segments of DNA into yeast using artificial chromosome (YAC) vectors. PFGE and blot hybridization analysis of Arabidopsis and carrot DNA-containing YACs indicated that both unique and highly repeated DNA sequences were represented in these libraries.

MeSH Terms
Chloroplasts/analysis Chromosomes, Fungal Cloning, Molecular/methods DNA, Recombinant Electrophoresis, Agar Gel/methods Gene Library Molecular Weight Plants/genetics Plants, Edible/genetics Restriction Mapping/methods Saccharomyces cerevisiae/genetics
Chemicals
DNA, Recombinant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guzmán P
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Ecker J R
References (29)
29 references, click to expand
  1. Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
    Am J Hum Genet. 1980 May;32(3):314-31 PMID: 6247908
  2. Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6812-6 PMID: 6093122
  3. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  4. Strategies for mapping and cloning macroregions of mammalian genomes.
    Methods Enzymol. 1987;151:461-89 PMID: 2828836
  5. Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene.
    Nature. 1986 Oct 16-22;323(6089):646-50 PMID: 3773991
  6. A fission yeast chromosome can replicate autonomously in mouse cells.
    Cell. 1987 Jul 31;50(3):391-403 PMID: 3475186
  7. A Genetic Map of Lettuce (Lactuca sativa L.) with Restriction Fragment Length Polymorphism, Isozyme, Disease Resistance and Morphological Markers.
    Genetics. 1987 Jun;116(2):331-7 PMID: 17246387
  8. Physical mapping of large DNA by chromosome fragmentation.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6027-31 PMID: 3045811
  9. Arabidopsis thaliana.
    Annu Rev Genet. 1987;21:93-111 PMID: 3327474
  10. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
    Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 PMID: 6379602
  11. Plant hormone mutants.
    Trends Genet. 1988 Jun;4(6):157-62 PMID: 2855559
  12. Stable transformation of maize after gene transfer by electroporation.
    Nature. 1986 Feb 27-Mar 5;319(6056):791-3 PMID: 3005872
  13. The abdominal region of the bithorax complex.
    Cell. 1985 Nov;43(1):81-96 PMID: 3935319
  14. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.
    Science. 1987 May 15;236(4803):806-12 PMID: 3033825
  15. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.
    Nucleic Acids Res. 1987 Jun 11;15(11):4481-9 PMID: 3295780
  16. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  17. Separation of large DNA molecules by contour-clamped homogeneous electric fields.
    Science. 1986 Dec 19;234(4783):1582-5 PMID: 3538420
  18. A long range restriction map of the pseudoautosomal region by partial digest PFGE analysis from the telomere.
    Nucleic Acids Res. 1988 Jun 24;16(12):5361-77 PMID: 2838815
  19. Inhibition of gene expression in plant cells by expression of antisense RNA.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5372-6 PMID: 16593734
  20. Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci.
    J Mol Biol. 1979 Aug 15;132(3):387-410 PMID: 533897
  21. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
    Nucleic Acids Res. 1987 Oct 12;15(19):7865-76 PMID: 2959907
  22. Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences.
    Genetics. 1986 Apr;112(4):887-98 PMID: 17246322
  23. Structural organization and functional analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.
    Mol Cell Biol. 1988 Feb;8(2):754-63 PMID: 2832735
  24. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856-60 PMID: 2901107
  25. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.
    J Mol Biol. 1983 Jul 25;168(1):17-33 PMID: 6410077
  26. An electrophoretic karyotype of Neurospora crassa.
    Mol Cell Biol. 1988 Apr;8(4):1469-73 PMID: 2967910
  27. The molecular structure of centromeres and telomeres.
    Annu Rev Biochem. 1984;53:163-94 PMID: 6383193
  28. Transformation of yeast spheroplasts without cell fusion.
    Anal Biochem. 1987 Jun;163(2):391-7 PMID: 3310730
  29. Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster.
    Cell. 1985 Aug;42(1):309-16 PMID: 2990728
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.23.11091
Published
1988-12-09
Pages
11091-105
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338998
Subset
IM
Grants
NIGMS NIH HHS · GM38894 · United States
External Links
PubMed source DOI: 10.1093/nar/16.23.11091 PMC Full Text View journal details
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