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PMID: 2990728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster.

Cell ·Vol. 42 ·No. 1 ·1985-08-00 ·Pages 309-16

Kuner JM, Nakanishi M, Ali Z, Drees B, Gustavson E, Theis J, Kauvar L, Kornberg T, O'Farrell PH

Abstract

The engrailed gene acts early in Drosophila embryogenesis and plays an essential role in the processes that establish and maintain the repeating segmental pattern. To begin molecular analysis of the role of the engrailed gene in embryonic pattern formation, we used a chromosomal walk to clone genomic sequences that encompass the locus, and have physically mapped the positions of 15 engrailed mutations. The positions of engrailed rearrangement mutations indicate that the engrailed complementation unit includes a minimum of 70 kb. The locus can be divided into two regions. Rearrangement mutations interrupting the centromere proximal 50 kb of the locus result in embryonic lethality while mutants altered in the distal 20 kb of the locus survive to show morphological abnormalities in several adult segments. It appears that long-range cis interactions play a role in the function of the engrailed gene.

MeSH Terms
Alleles Animals Chromosome Mapping Cloning, Molecular DNA/genetics DNA Restriction Enzymes Drosophila melanogaster/embryology,genetics,growth & development Genetic Complementation Test Mutation Translocation, Genetic
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kuner J M
Nakanishi M
Ali Z
Drees B
Gustavson E
Theis J
Kauvar L
Kornberg T
O'Farrell P H
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/s0092-8674(85)80126-4
Published
1985-08-00
Pages
309-16
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2753415
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037193 · United States
NIGMS NIH HHS · R01 GM037193-10 · United States
External Links
PubMed source DOI: 10.1016/s0092-8674(85)80126-4 PMC Full Text View journal details
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