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PMID: 3045824 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6792-6

Dike LE, Farmer SR

Abstract

A methylcellulose suspension system that prevents cell-surface contact with the substrate was used to study the role of cell adhesion in the regulation of proliferation. The nonadhesive conditions established by suspension culture cause BALB/c 3T3 (A31) cells to enter a G0 state of growth arrest within 48 hr as defined by an inhibition of DNA synthesis and a suppression of c-myc and histone mRNA expression. The adhesion of these suspension-arrested cells rapidly induces c-fos, c-myc, and actin gene expression. This stimulation did not depend on the presence of serum since the adhesion of suspension-arrested cells, in the absence of serum, also induced the expression of c-fos and c-myc mRNAs. In addition, adhesion onto fibronectin increased the number of cells able to respond to epidermal growth factor and insulin and progress into S phase. These results indicate that adhesion of suspension-arrested cells activates the G0/G1 transition independent of growth factors.

MeSH Terms
Actins/genetics Animals Cell Adhesion DNA Replication Fibroblasts/metabolism Fibronectins/metabolism Gene Expression Regulation Growth Substances/genetics Insulin/metabolism Mice Mice, Inbred BALB C Oncogenes Transcription, Genetic
Chemicals
Actins Fibronectins Growth Substances Insulin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dike L E
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Farmer S R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6792
Published
1988-09-00
Pages
6792-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282064
Subset
IM
Grants
NIGMS NIH HHS · GM29630 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.18.6792 PMC Full Text View journal details
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