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PMID: 3034587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Orientation and molecular map position of the complement genes in the mouse MHC.

The EMBO journal ·Vol. 6 ·No. 2 ·1987-02-00 ·Pages 369-73

Müller U, Stephan D, Philippsen P, Steinmetz M

Abstract

Over the past few years six gene clusters have been isolated from the major histocompatibility complex (MHC) of the BALB/c mouse encompassing a total of 1600 kb of DNA and 48 genes. The molecular distances between these gene clusters and the orientation of four of the six clusters on chromosome 17 is not known. Here we use pulse-field gradient gels and Southern blot hybridization to establish large-scale genomic restriction maps covering several hundreds of kb surrounding the three gene clusters located in the K, I, S, and D regions of the MHC. Comparison of the maps orients the complement gene clusters in the S region with the 21-OHB gene pointing towards the K end and the C2 gene pointing towards the D end of the MHC. The distances between the E alpha and 21-OHB genes is 430 kb and between the C2 and TNF-alpha genes at least 420 kb.

MeSH Terms
Animals Complement System Proteins/genetics DNA Restriction Enzymes Genes Lymphocytes/immunology Major Histocompatibility Complex Mice Mice, Inbred BALB C Spleen/immunology
Chemicals
Complement System Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Müller U
Stephan D
Philippsen P
Steinmetz M
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22 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-02-00
Pages
369-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553405
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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