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PMID: 3023818 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple C4/Slp genes distinguished by expression after transfection.

Molecular and cellular biology ·Vol. 6 ·No. 1 ·1986-01-00 ·Pages 134-41

Robins DM, Malissen M, Hood L, Ferreira A, Walthall D, Mitchell M

Abstract

The S region of the murine major histocompatibility complex contains two closely related genes: C4, encoding the fourth component of complement, and Slp, encoding sex-limited protein. We cloned these genes from a cosmid library of the B10.W7R strain that does not show androgen regulation of the Slp protein. Restriction site polymorphisms revealed at least four C4-like genes within the Sw7 locus, indicating evolutionary amplification of this region. Transfection of these genes into L cells resulted in expression, processing, and secretion of immunologically correct C4 and Slp proteins. At least two different Slp genes and one C4 gene were capable, after transfection, of expressing C4 and Slp indistinguishable from macrophage-derived protein. A third Slp gene exists within this locus whose recombinant cognate did not express in L cells. Thus, the B10.W7R S region includes one C4 gene and at least three Slp-like genes.

MeSH Terms

Animals Blood Proteins/biosynthesis,genetics Complement C4/biosynthesis,genetics Cosmids DNA/metabolism DNA Restriction Enzymes Genes Major Histocompatibility Complex Mice Nucleic Acid Hybridization Transfection

Chemicals

Blood Proteins C4a protein, mouse Complement C4 DNA DNA Restriction Enzymes

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Robins D M

Malissen M

Hood L

Ferreira A

Walthall D

Mitchell M

References (24)

24 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.6.1.134-141.1986

Published

1986-01-00

Pages

134-41

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC367492

Subset

Grants

NIAID NIH HHS · AI13224 · United States

NIGMS NIH HHS · GM31546 · United States

External Links

PubMed source DOI: 10.1128/mcb.6.1.134-141.1986 PMC Full Text View journal details

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Multiple C4/Slp genes distinguished by expression after transfection.

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