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PMID: 3033297 Published · ppublish English Journal Article

Continued expression of a poly(A)+ transcript of herpes simplex virus type 1 in trigeminal ganglia of latently infected mice.

Journal of virology ·Vol. 61 ·No. 5 ·1987-05-00 ·Pages 1700-3

Puga A, Notkins AL

Abstract

Radioactively labeled cDNAs were prepared by using as the template poly(A)+ mRNA from trigeminal ganglia of mice latently infected with herpes simplex virus type 1. These cDNAs were used as hybridization probes for Southern blots of cloned herpes simplex virus type 1 DNA fragments. Specific hybridization to fragments from the terminal repetition of the L segment was detected with probes derived from mRNAs obtained as early as 3 weeks and as late as 17 months postinoculation. Fine mapping of the region of hybridization showed that the viral transcripts originated from DNA sequences coding for the immediate-early gene IE-1 (alpha-0). These results indicate that IE-1, or an as yet unidentified gene colinear with it, is continuously expressed during latency.

MeSH Terms
Animals Gene Expression Regulation Herpes Simplex/genetics Mice Poly A/metabolism RNA, Messenger/genetics RNA, Viral/genetics Simplexvirus/genetics Time Factors Trigeminal Ganglion/microbiology Trigeminal Nerve/microbiology Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Proteins Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Puga A
Notkins A L
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19 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.5.1700-1703.1987
Published
1987-05-00
Pages
1700-3
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254159
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.5.1700-1703.1987 PMC Full Text View journal details
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