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PMID: 6324121 Published · ppublish English Journal Article

A 3' co-terminal family of mRNAs from the herpes simplex virus type 1 short region: two overlapping reading frames encode unrelated polypeptide one of which has highly reiterated amino acid sequence.

Nucleic acids research ·Vol. 12 ·No. 5 ·1984-03-12 ·Pages 2473-87

Rixon FJ, McGeoch DJ

Abstract

We have used DNA sequencing, mRNA mapping and in vitro translation to characterise three partially overlapping genes in the genome of herpes simplex virus (HSV) type 1. These genes specify three mRNAs with distinct 5' termini but a common 3' terminus, the longest of which is immediate-early (IE) mRNA-5. The 12,000 MW (12K) IE polypeptide encoded by IEmRNA-5 is translated from an 88 codon open reading frame, leaving a 1200 base 3' non-translated region. The second mRNA (mRNA-B) is initiated within the coding sequence of IEmRNA-5, and encodes a 21K polypeptide. The 12K and 21K polypeptide coding regions do not overlap. The third mRNA (mRNA-C) is initiated within the coding region of mRNA-B, and encodes a 33K polypeptide. The reading frame for 33K has a 110 codon out-of-frame overlap with the 21K reading frame. This is the first instance of overlapping genes described for HSV. The 21K polypeptide is thought to be a DNA binding protein and is remarkable for an array of 24 tandem repeats of the sequence X/Pro/Arg (where X represents predominantly Glu, Asp, Thr, Ser or Val) in its C-terminal portion. This array, which occupies most of the region of overlap with 33K, can vary in repeat number between virus strains.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Cricetinae DNA Restriction Enzymes Genes, Viral Genetic Vectors Kidney Molecular Weight Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Simplexvirus/genetics
Chemicals
RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rixon F J
McGeoch D J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.5.2473
Published
1984-03-12
Pages
2473-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318677
Subset
IM
Databases
GENBANK
L00036, L00037, M12354, M12506, X00428
External Links
PubMed source DOI: 10.1093/nar/12.5.2473 PMC Full Text View journal details
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