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PMID: 3032984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation by aldosterone of Na+,K+-ATPase mRNAs, protein synthesis, and sodium transport in cultured kidney cells.

The Journal of cell biology ·Vol. 104 ·No. 5 ·1987-05-00 ·Pages 1231-7

Verrey F, Schaerer E, Zoerkler P, Paccolat MP, Geering K, Kraehenbuhl JP, Rossier BC

Abstract

Transepithelial Na+ reabsorption across tight epithelia is regulated by aldosterone. Mineralocorticoids modulate the expression of a number of proteins. Na+,K+-ATPase has been identified as an aldosterone-induced protein (Geering, K., M. Girardet, C. Bron, J. P. Kraehenbuhl, and B. C. Rossier, 1982, J. Biol. Chem., 257:10338-10343). Using A6 cells (kidney of Xenopus laevis) grown on filters we demonstrated by Northern blot analysis that the induction of Na+,K+-ATPase was mainly mediated by a two- to fourfold accumulation of both alpha- and beta-subunit mRNAs. The specific competitor spironolactone decreased basal Na+ transport, Na+,K+-ATPase mRNA, and the relative rate of protein biosynthesis, and it blocked the response to aldosterone. Cycloheximide inhibited the aldosterone-dependent sodium transport but did not significantly affect the cytoplasmic accumulation of Na+,K+-ATPase mRNA induced by aldosterone.

MeSH Terms
Aldosterone/pharmacology Animals Biological Transport, Active/drug effects Cell Line Cycloheximide/pharmacology Kidney/drug effects,metabolism Macromolecular Substances Nucleic Acid Hybridization Protein Biosynthesis/drug effects RNA, Messenger/drug effects,genetics Sodium/metabolism Sodium-Potassium-Exchanging ATPase/biosynthesis,genetics Xenopus laevis
Chemicals
Macromolecular Substances RNA, Messenger Aldosterone Cycloheximide Sodium Sodium-Potassium-Exchanging ATPase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Verrey F
Schaerer E
Zoerkler P
Paccolat M P
Geering K
Kraehenbuhl J P
Rossier B C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.104.5.1231
Published
1987-05-00
Pages
1231-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114475
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.104.5.1231 PMC Full Text View journal details
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