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PMID: 3031457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

DNA sequence and transcript mapping of MOD5: features of the 5' region which suggest two translational starts.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 185-91

Najarian D, Dihanich ME, Martin NC, Hopper AK

Abstract

A mutation in the yeast nuclear gene MOD5 drastically reduces the biosynthesis of the modified base isopentenyladenosine in tRNAs located in different cellular compartments: the mitochondria and the nucleus or cytoplasm. Several lines of evidence strongly suggest that MOD5 is the structural gene encoding the tRNA-modifying enzyme delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase. DNA sequence analysis of MOD5 reveals an open reading frame of 428 amino acids. A set of mRNAs heterogeneous at both the 5' and 3' termini are transcribed from this gene. Although all of these transcripts initiate upstream of the first AUG codon of the open reading frame, a subset has an extremely short (greater than or equal to 1 base) 5' leader. Furthermore, in positions important for efficient initiation of translation and generally occupied by purines, this first AUG codon is flanked by a U (position -3) and a C (position +4). It is possible that two proteins, one with an amino-terminal extension of basic charge, could be generated from the MOD5 gene via differential translational starts.

MeSH Terms
Alkyl and Aryl Transferases Amino Acid Sequence Base Sequence Cell Nucleus/metabolism DNA Restriction Enzymes Genes Genes, Fungal Mutation Protein Biosynthesis RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription, Genetic Transferases/genetics
Chemicals
RNA, Messenger Saccharomyces cerevisiae Proteins Transferases Alkyl and Aryl Transferases MOD5 protein, S cerevisiae tRNA isopentenyltransferase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Najarian D
Dihanich M E
Martin N C
Hopper A K
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.185-191.1987
Published
1987-01-00
Pages
185-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365055
Subset
IM
Databases
GENBANK
M15991
External Links
PubMed source DOI: 10.1128/mcb.7.1.185-191.1987 PMC Full Text View journal details
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