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PMID: 3030266 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

6-beta-Iodopenicillanate as a probe for the classification of beta-lactamases.

The Biochemical journal ·Vol. 239 ·No. 3 ·1986-11-01 ·Pages 575-80

De Meester F, Frère JM, Waley SG, Cartwright SJ, Virden R, Lindberg F

Abstract

An inactivator of serine beta-lactamases, 6 beta-iodopenicillanate, can be utilized as a probe in the classification of beta-lactamases. It is a substrate for class-B Zn2+-containing beta-lactamase II. Although it inactivates enzymes from both classes A and C, it is much more efficient for the former group, with which it sometimes interacts following a branched pathway. On the basis of these observations, predictions are made concerning the class to which several enzymes belong.

MeSH Terms
Hydrogen-Ion Concentration Kinetics Penicillanic Acid/pharmacology Spectrophotometry Thiazines beta-Lactamase Inhibitors beta-Lactamases/classification
Chemicals
Thiazines beta-Lactamase Inhibitors 2,3-dihydro-2,2-dimethyl-1,4-thiazine-3,6-dicarboxylate Penicillanic Acid 6-iodopenicillanic acid beta-Lactamases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
De Meester F
Frère J M
Waley S G
Cartwright S J
Virden R
Lindberg F
References (29)
29 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2390575
Published
1986-11-01
Pages
575-80
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147325
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2390575 PMC Full Text View journal details
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