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PMID: 2988516 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.

The Biochemical journal ·Vol. 228 ·No. 1 ·1985-05-15 ·Pages 241-8

Joris B, De Meester F, Galleni M, Reckinger G, Coyette J, Frere JM, Van Beeumen J

Abstract

The beta-lactamase of Enterobacter cloacae P99 consists of one polypeptide chain of Mr 39000 devoid of disulphide bridges and free thiol groups. It contains an unusually high proportion of tyrosine and tryptophan. The N-terminal sequence exhibits overlaps with the tryptic peptide obtained after labelling the active site with 6 beta-iodopenicillanate. The active-site serine residue is at position 64. The homology with the chromosomal beta-lactamase of Escherichia coli K 12 (ampC gene) is lower within the 25 residues of the N-terminal portion than around the active-site serine residue. The P99 beta-lactamase is inactivated by 6 beta-bromo- and 6 beta-iodo-penicillanate, with a second-order rate constant of 110-140M-1 X s-1 at 30 degrees C and pH 7.0, a value that is much lower than that observed with class-A beta-lactamases.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Enterobacter/enzymology Enterobacteriaceae/enzymology Kinetics Penicillanic Acid/metabolism Spectrophotometry Tryptophan/analysis beta-Lactamase Inhibitors beta-Lactamases
Chemicals
Amino Acids beta-Lactamase Inhibitors brobactam Penicillanic Acid Tryptophan 6-iodopenicillanic acid beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Joris B
De Meester F
Galleni M
Reckinger G
Coyette J
Frere J M
Van Beeumen J
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22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2280241
Published
1985-05-15
Pages
241-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144975
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2280241 PMC Full Text View journal details
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