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PMID: 3025875 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural features of cartilage matrix protein deduced from cDNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 2 ·1987-01-00 ·Pages 464-8

Argraves WS, Deák F, Sparks KJ, Kiss I, Goetinck PF

Abstract

cDNAs encoding the Mr 54,000 chicken cartilage matrix protein (CMP) were selected from a cartilage cDNA expression library by immunological means. Antibodies elicited against insert-encoded protein purified from one of the clones reacted specifically with chicken CMP in immunoblots of total cartilage extract, providing positive identification of the cDNA clones isolated. The cDNAs detect a 3.4-kilobase transcript that was present in sternal cartilage and in cartilaginous but not in precartilaginous embryonic limb tissues. The cDNAs code for 416 amino acids of the chicken CMP, including its COOH terminus. There are two striking features in the deduced CMP amino acid sequence: first, it contains a region with significant homologies to repeat sequences in the precursor for epidermal growth factor; and second, it is made up of two large homologous repeat sequences. These results provide the first detailed structural information on the CMP and establish it as a developmentally regulated marker of cartilage differentiation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cartilage/embryology,metabolism Cells, Cultured Chick Embryo DNA/metabolism DNA Transposable Elements Extracellular Matrix Proteins Fluorescent Antibody Technique Glycoproteins/genetics Matrilin Proteins RNA, Messenger/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements Extracellular Matrix Proteins Glycoproteins Matrilin Proteins RNA, Messenger DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Argraves W S
Deák F
Sparks K J
Kiss I
Goetinck P F
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.2.464
Published
1987-01-00
Pages
464-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304228
Subset
IM
Grants
NICHD NIH HHS · HD 09174 · United States
NICHD NIH HHS · HD 22016 · United States
Databases
GENBANK
M14792
External Links
PubMed source DOI: 10.1073/pnas.84.2.464 PMC Full Text View journal details
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