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PMID: 3014533 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vitro regulation of cartilage matrix assembly by a Mr 54,000 collagen-binding protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 14 ·1986-07-00 ·Pages 5126-30

Chandrasekhar S, Laurie GW, Cannon FB, Martin GR, Kleinman HK

Abstract

In cartilage, type II collagen is present as thin, short, randomly oriented fibrils. In vitro, however, type II collagen forms fibrils of large diameter, indicating that additional factors may be involved in the regulation of collagen fibril formation. We have examined extracts of a cartilage-producing tumor for the presence of collagen-binding proteins. In addition to fibronectin and link protein, a Mr 54,000 protein was found to bind to collagen fibrils as well as to native and denatured type II collagen. Immunological studies using antibody against the protein indicate that it is a cartilage matrix protein, not present in bone or in several other tissues. In vitro studies show that the Mr 54,000 protein in combination with cartilage proteoglycan decreases the rate of type II fibril formation and causes the fibrils to be of small diameter (24 +/- 8 nm). These studies indicate that complexes between collagen and proteoglycans mediated by this protein may regulate the assembly of cartilage matrix.

MeSH Terms
Animals Carrier Proteins/isolation & purification,physiology Cartilage/metabolism Collagen/metabolism Extracellular Matrix/metabolism Molecular Weight Proteoglycans/metabolism Rats Receptors, Cell Surface/isolation & purification,physiology Receptors, Collagen Sarcoma, Experimental/analysis
Chemicals
Carrier Proteins Proteoglycans Receptors, Cell Surface Receptors, Collagen Collagen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chandrasekhar S
Laurie G W
Cannon F B
Martin G R
Kleinman H K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.14.5126
Published
1986-07-00
Pages
5126-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323903
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.14.5126 PMC Full Text View journal details
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