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PMID: 3459153 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Link protein cDNA sequence reveals a tandemly repeated protein structure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3761-5

Doege K, Hassell JR, Caterson B, Yamada Y

Abstract

Link protein stabilizes the cartilage proteoglycan/hyaluronic acid aggregate by binding to both components. We screened a cDNA library prepared from rat chondrosarcoma mRNA in the lambda gt11 expression vector with monoclonal antibodies and polyclonal antisera to link protein. We obtained a clone for two-thirds of the link protein cDNA and identified it based on its deduced amino acid sequence. There are four RNA transcripts for link protein, ranging from 1.5 to 5.5 kilobases in size. The deduced amino acid sequence for link protein shows two domains of 100 residues each, which share 44% homology; within each domain is a 19-residue stretch 74% homologous with its counterpart. This structure indicates that link protein may have been formed by gene duplication.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Extracellular Matrix Proteins Protein Conformation Proteins/genetics Proteoglycans/genetics Rats
Chemicals
Extracellular Matrix Proteins Proteins Proteoglycans link protein DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Doege K
Hassell J R
Caterson B
Yamada Y
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3761
Published
1986-06-00
Pages
3761-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323603
Subset
IM
Grants
NIDCR NIH HHS · 1 F32 DEO5397-01 · United States
Databases
GENBANK
J03201, M13191, M22336, M22337, M22338, M22339, M22340
External Links
PubMed source DOI: 10.1073/pnas.83.11.3761 PMC Full Text View journal details
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