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PMID: 3023821 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons.

Molecular and cellular biology ·Vol. 6 ·No. 1 ·1986-01-00 ·Pages 168-82

Loeb DD, Padgett RW, Hardies SC, Shehee WR, Comer MB, Edgell MH, Hutchison CA

Abstract

The complete nucleotide sequence of a 6,851-base pair (bp) member of the L1Md repetitive family from a selected random isolate of the BALB/c mouse genome is reported here. Five kilobases of the element contains two overlapping reading frames of 1,137 and 3,900 bp. The entire 3,900-bp frame and the 3' 600 bp of the 1,137-bp frame, when compared with a composite consensus primate L1 sequence, show a ratio of replacement to silent site differences characteristic of protein coding sequences. This more closely defines the protein coding capacity of this repetitive family, which was previously shown to possess a large open reading frame of undetermined extent. The relative organization of the 1,137- and 3,900-bp reading frames, which overlap by 14 bp, bears resemblance to protein-coding, mobile genetic elements. Homology can be found between the amino acid sequence of the 3,900-bp frame and selected domains of several reverse transcriptases. The 5' ends of the two L1Md elements described in this report have multiple copies, 4 2/3 copies and 1 2/3 copy, of a 208-bp direct tandem repeat. The sequence of this 208-bp element differs from the sequence of a previously defined 5' end for an L1Md element, indicating that there are at least two different 5' end motifs for L1Md.

MeSH Terms
Amino Acid Sequence Animals Base Composition Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Genes Globins/genetics Mice Mice, Inbred BALB C Phylogeny Repetitive Sequences, Nucleic Acid Retroviridae/genetics
Chemicals
DNA Transposable Elements Globins DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Loeb D D
Padgett R W
Hardies S C
Shehee W R
Comer M B
Edgell M H
Hutchison C A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.1.168-182.1986
Published
1986-01-00
Pages
168-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367496
Subset
IM
Grants
NIAID NIH HHS · AI08998 · United States
NIGMS NIH HHS · GM21313 · United States
Databases
GENBANK
M13002
External Links
PubMed source DOI: 10.1128/mcb.6.1.168-182.1986 PMC Full Text View journal details
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