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PMID: 3022287 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Oxygen-dependent mutagenesis in Escherichia coli lacking superoxide dismutase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 21 ·1986-11-00 ·Pages 8268-72

Farr SB, D'Ari R, Touati D

Abstract

Escherichia coli double mutants (sodA sodB) completely lacking superoxide dismutase (SOD) have greatly enhanced mutation rates during aerobic growth. Single mutants lacking manganese SOD (MnSOD) but possessing iron SOD (FeSOD) have a smaller increase, and single mutants lacking FeSOD but possessing MnSOD do not show such an increase. The enhancement of mutagenesis is completely dependent on the presence of oxygen, and treatments that increase the flux of superoxide radicals produce even higher levels of mutagenesis. The presence of a plasmid overproducing either form of SOD reduces the level of mutagenesis to that of wild type, showing that the O2-dependent enhancement results from a lack of SOD. The enhancement of mutagenesis is RecA-independent, and a complete lack of SOD does not induce the SOS response during aerobic growth. However, the enhanced mutagenesis in aerobically grown sodA sodB mutants is largely dependent on functional exonuclease III, suggesting that the increased flux of superoxide radicals results in DNA lesions that can be acted on by this enzyme, leading to mutations.

MeSH Terms
DNA Damage Escherichia coli/genetics Exodeoxyribonucleases/physiology Free Radicals Mutation Oxygen/pharmacology SOS Response, Genetics Superoxide Dismutase/deficiency,genetics,physiology Superoxides/metabolism beta-Galactosidase/genetics
Chemicals
Free Radicals Superoxides Superoxide Dismutase Exodeoxyribonucleases exodeoxyribonuclease III beta-Galactosidase Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Farr S B
D'Ari R
Touati D
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.21.8268
Published
1986-11-00
Pages
8268-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386909
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.21.8268 PMC Full Text View journal details
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