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PMID: 30139799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reconstitution of mammalian cleavage factor II involved in 3' processing of mRNA precursors.

RNA (New York, N.Y.) ·Vol. 24 ·No. 12 ·2018-00-00 ·Pages 1721-1737

Schäfer P, Tüting C, Schönemann L, Kühn U, Treiber T, Treiber N, Ihling C, Graber A, Keller W, Meister G, Sinz A, Wahle E

Abstract

Cleavage factor II (CF II) is a poorly characterized component of the multiprotein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactions, whereas hClp1 by itself is not. Pcf11 moderately stimulates the RNA 5' kinase activity of hClp1; the kinase activity is dispensable for RNA cleavage. CF II binds RNA with nanomolar affinity. Binding is mediated mostly by the two zinc fingers in the C-terminal region of hPcf11. RNA is bound without pronounced sequence-specificity, but extended G-rich sequences appear to be preferred. We discuss the possibility that CF II contributes to the recognition of cleavage/polyadenylation substrates through interaction with G-rich far-downstream sequence elements.

Keywords
3′ end formation Clp1 Pcf11 mRNA processing polyadenylation
MeSH Terms
Binding Sites Multiprotein Complexes/chemistry,genetics Nuclear Proteins/chemistry,genetics Phosphotransferases/chemistry,genetics Polyadenylation/genetics Protein Binding Protein Multimerization RNA Precursors/chemistry,genetics Sequence Homology, Amino Acid Transcription Factors/chemistry,genetics mRNA Cleavage and Polyadenylation Factors/chemistry,genetics
Chemicals
Multiprotein Complexes Nuclear Proteins Pcf11 protein, human RNA Precursors Transcription Factors mRNA Cleavage and Polyadenylation Factors CLP1 protein, human Phosphotransferases
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Schäfer Peter
Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
Tüting Christian
Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
Schönemann Lars
Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
Kühn Uwe
Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
Treiber Thomas
Biochemistry Center Regensburg, Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany.
Treiber Nora
Biochemistry Center Regensburg, Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany.
Ihling Christian
Institute of Pharmacy, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
Graber Anne
Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany. | Biozentrum, University of Basel, CH-4056 Basel, Switzerland.
Keller Walter
Biozentrum, University of Basel, CH-4056 Basel, Switzerland.
Meister Gunter
Biochemistry Center Regensburg, Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany.
Sinz Andrea
Institute of Pharmacy, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
Wahle Elmar ORCID
Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, 06099 Halle, Germany.
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1469-9001
DOI
10.1261/rna.068056.118
Published
2018-00-00
Epub
2018-00-23
Pages
1721-1737
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC6239180
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.068056.118 PMC Full Text View journal details
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