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PMID: 3010243 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the major heat shock gene of Drosophila melanogaster in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 14 ·No. 8 ·1986-04-25 ·Pages 3587-601

de Banzie JS, Sinclair L, Lis JT

Abstract

A copy of the gene which encodes the major heat shock protein (hsp70) of D. melanogaster was integrated in both orientations into the genome of S. cerevisiae at the leu2 locus. The level of transcript from the D. melanogaster gene was measured under both normal conditions and conditions which are known to give rise to the heat shock response in S. cerevisiae. In both orientations the D. melanogaster gene gave rise to an abundant transcript in uninduced cells. The level of this transcript was increased transiently on heat shock, peaking after about 30 min at the elevated temperature. The average induction observed was around 5-fold. Although the D. melanogaster gene is heat inducible in S. cerevisiae, the transcripts are initiated at several sites which lie between 10 and 40 base pairs downstream of the initiation site in D. melanogaster. Thus, the transcriptional apparatus of S. cerevisiae appears to recognize the promoter and regulatory elements of the D. melanogaster major heat shock gene, although the manner in which transcription is initiated differs between the two species.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes Drosophila melanogaster/genetics Genes Genes, Fungal Heat-Shock Proteins/genetics Hot Temperature Nucleic Acid Hybridization Plasmids Saccharomyces cerevisiae/genetics Species Specificity Transcription, Genetic
Chemicals
Heat-Shock Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
de Banzie J S
Sinclair L
Lis J T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.8.3587
Published
1986-04-25
Pages
3587-601
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339795
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.8.3587 PMC Full Text View journal details
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