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PMID: 3009887 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.

Journal of virology ·Vol. 58 ·No. 3 ·1986-06-00 ·Pages 790-6

Hey TD, Richards OC, Ehrenfeld E

Abstract

The poliovirus RNA polymerase, 3Dpol, was used to synthesize RNA in vitro in the presence of a host factor preparation from uninfected HeLa cells and poliovirion RNA as the template. The transcription products included molecules approximately twice the length of the template, apparently resulting from hairpin formation and template-directed elongation, as previously reported (D. C. Young, D. M. Tuschall, and J. B. Flanegan, J. Virol. 54:256-264, 1985). Other polyadenylated template RNAs also yielded products that were twice the length of the template. The polarity of the products synthesized from plus-strand poliovirus RNA template was analyzed by Southern blotting using labeled product RNA to probe single-stranded poliovirus DNAs cloned into M13 vectors. The results demonstrated that host factor-mediated polymerase products contain newly synthesized plus-strand sequences as well as the expected minus-strand sequences. Polymerase products primed with oligo(U) were all of minus-strand polarity.

MeSH Terms
Base Sequence Carrier Proteins/physiology DNA-Directed RNA Polymerases/analysis Integration Host Factors Nucleic Acid Hybridization Poliovirus/metabolism Poly U/metabolism RNA, Viral/biosynthesis Templates, Genetic Viral Core Proteins Viral Proteins/immunology,metabolism
Chemicals
Carrier Proteins Integration Host Factors RNA, Viral Viral Core Proteins Viral Proteins Poly U DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hey T D
Richards O C
Ehrenfeld E
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36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.3.790-796.1986
Published
1986-06-00
Pages
790-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252985
Subset
IM
Grants
NIAID NIH HHS · AI-17386 · United States
External Links
PubMed source DOI: 10.1128/JVI.58.3.790-796.1986 PMC Full Text View journal details
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