Two unique restriction endonucleases from Neisseria lactamica.

Nucleic acids research ·Vol. 14 ·No. 5 ·1986-03-11 ·Pages 1991-9

Qiang BQ, Schildkraut I

Abstract

Two new site-specific endonucleases, N1a III and N1a IV, have been isolated from Neisseria lactamica. N1a III recognizes the sequence, CATG, and cleaves 3' of the sequence to produce a four base 3' extension. N1a IV recognizes the sequence, GGNNCC, and cleaves between the two N's to produce blunt ended fragments.

MeSH Terms

Base Sequence Buffers DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type II Site-Specific Neisseria/enzymology Substrate Specificity

Chemicals

Buffers DNA Restriction Enzymes endodeoxyribonuclease NlaIII endodeoxyribonuclease NlaIV Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Qiang B Q

Schildkraut I

References (9)

9 references, click to expand

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Characterization of a site-specific restriction endonuclease SphI from Streptomyces phaeochromogenes.
Gene. 1980 Jun;10(1):39-46 PMID: 6250945
Restriction and modification enzymes and their recognition sequences.
Nucleic Acids Res. 1985;13 Suppl:r165-200 PMID: 2987885
Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs.
Gene. 1980 Sep;10(4):357-70 PMID: 6253357
Two new restriction endonucleases from Proteus vulgaris.
Nucleic Acids Res. 1981 Sep 25;9(18):4525-36 PMID: 6272209
Sequence-specific DNA modification in Neisseria gonorrhoeae.
J Bacteriol. 1983 Sep;155(3):1324-32 PMID: 6411687
The specific non-symmetrical sequence recognized by restriction endonuclease MboII.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.5.1991

Published

1986-03-11

Pages

1991-9

Language

English

Region

England

NLM ID

0411011

PMCID

PMC339638

Subset

External Links

PubMed source DOI: 10.1093/nar/14.5.1991 PMC Full Text View journal details

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Two unique restriction endonucleases from Neisseria lactamica.

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