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PMID: 6411687 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence-specific DNA modification in Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 155 ·No. 3 ·1983-09-00 ·Pages 1324-32

Korch C, Hagblom P, Normark S

Abstract

Neisseria gonorrhoeae 82409/55(pJD1) is postulated to possess six DNA sequence-specific cytosine methyltransferases and one DNA sequence-specific N6-adenine methyltransferase. From the DNA sequencing of the plasmid pJD1 (manuscript in preparation) by a modification of the Maxam and Gilbert chemical cleavage procedure, the cytosine methylation specificities were demonstrated. Five of these methylating enzymes and their respective specificities are M . NgoI (formula; see text) does not methylate the cytosine of its recognition sequence, in agreement with a detected adenine modification. A biological implication of these different DNA methylating activities is discussed.

MeSH Terms
Base Sequence DNA, Bacterial/metabolism Methylation Methyltransferases/metabolism Neisseria gonorrhoeae/enzymology Plasmids Substrate Specificity
Chemicals
DNA, Bacterial Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Korch C
Hagblom P
Normark S
References (13)
13 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.3.1324-1332.1983
Published
1983-09-00
Pages
1324-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217831
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.155.3.1324-1332.1983 PMC Full Text View journal details
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