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PMID: 2997585 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic mapping of rpoD implicates the major sigma factor of Bacillus subtilis RNA polymerase in sporulation initiation.

Molecular & general genetics : MGG ·Vol. 201 ·No. 1 ·1985-00-00 ·Pages 88-95

Price CW, Doi RH

Abstract

We have mapped the chromosomal locus of rpoD, which encodes the major sigma factor of Bacillus subtilis RNA polymerase. The rpoD locus lay between aroD and lys, tightly linked to dnaE and inseparable from crsA. Marker order in this region was acf-aroD-dnaE-rpoD(crsA)-spoOG-lys. By transformation using cloned donor DNA from the rpoD region, we identified the gene immediately upstream of rpoD as dnaE, which coded for a 62,000 dalton protein essential for DNA replication. Both dnaE and rpoD were transcribed in the same direction, counterclockwise on the chromosome. The gene functions and organization in the rpoD region are thus similar to those of the E. coli sigma operon. We also used transformation to identify crsA47 as a mutation within the sigma coding region itself. The crsA alteration of sigma renders the sporulation process insensitive to glucose catabolite repression, and also restores sporulation ability to strains carrying early-blocked spoOE, spoOF, and spoOK mutations. Thus the major sigma factor and these spoO gene products directly or indirectly affect the same cellular function.

MeSH Terms
Bacillus subtilis/enzymology,genetics,physiology Chromosome Mapping Chromosomes, Bacterial/physiology DNA Restriction Enzymes DNA-Directed RNA Polymerases/genetics Genes Genes, Bacterial Genotype Mutation Plasmids Sigma Factor/genetics Species Specificity Spores, Bacterial/enzymology Transcription Factors/genetics
Chemicals
Sigma Factor Transcription Factors DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Price C W
Doi R H
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51 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00397991
Published
1985-00-00
Pages
88-95
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 19673 · United States
External Links
PubMed source DOI: 10.1007/BF00397991
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