Home LiteratureArticle Details
PMID: 2991919 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 16 ·1985-08-00 ·Pages 5360-4

Chang CC, Trosko JE, Kung HJ, Bombick D, Matsumura F

Abstract

The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (psrc-11). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60src, and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.

MeSH Terms
Animals Cell Communication Cell Transformation, Neoplastic Cells, Cultured Clone Cells DNA Restriction Enzymes Genes Intercellular Junctions/physiology Mice Mice, Inbred Strains Oncogene Protein pp60(v-src) Oncogenes Protein Kinase C Protein Kinases/genetics,metabolism Uracil Nucleotides/metabolism Viral Proteins/genetics,physiology
Chemicals
Uracil Nucleotides Viral Proteins Protein Kinases Oncogene Protein pp60(v-src) Protein Kinase C DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chang C C
Trosko J E
Kung H J
Bombick D
Matsumura F
References (38)
38 references, click to expand
  1. Elimination of metabolic cooperation in Chinese hamster cells by a tumor promoter.
    Science. 1979 Nov 30;206(4422):1089-91 PMID: 493994
  2. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  3. Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidylinositol turnover.
    J Biol Chem. 1980 Mar 25;255(6):2273-6 PMID: 7358670
  4. Inhibition of intercellular communication in Chinese hamster V79 cells by teleocidin.
    Gan. 1982 Dec;73(6):874-8 PMID: 6819176
  5. Patterns of cell communication and differentiation in SV40 transformed human keratinocytes.
    J Cell Physiol. 1981 Oct;109(1):153-9 PMID: 6276417
  6. Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factor.
    Nature. 1983 Oct 13-19;305(5935):605-8 PMID: 6312326
  7. Phenotypic correction of the defect in xeroderma pigmentosum cells after fusion with isolated cytoplasts.
    Exp Cell Res. 1982 Jul;140(1):119-25 PMID: 7106197
  8. Transformation by Rous sarcoma virus: effects of src gene expression on the synthesis and phosphorylation of cellular polypeptides.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5212-6 PMID: 228282
  9. Decreased incidence of gap junctions between Chinese hamster V-79 cells upon exposure to the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate.
    Exp Cell Res. 1982 Jun;139(2):329-40 PMID: 7084321
  10. Permeability of junctions between animal cells. Intercellular transfer of nucleotides but not of macromolecules.
    Exp Cell Res. 1977 Jan;104(1):153-63 PMID: 836400
  11. Comparison of contact-mediated communication in normal and transformed human cells in culture.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4476-80 PMID: 270694
  12. Tumor promoters inhibit metabolic cooperation in cocultures of epidermal and 3T3 cells.
    Biochem Biophys Res Commun. 1979 Nov 28;91(2):395-401 PMID: 518638
  13. Phorbol ester tumor promoter affects the mouse epidermal gap junctions.
    Cancer Lett. 1984 Apr;22(3):343-50 PMID: 6713375
  14. Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2117-21 PMID: 6326105
  15. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  16. Transforming protein of avian sarcoma virus UR2 is associated with phosphatidylinositol kinase activity: possible role in tumorigenesis.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2728-32 PMID: 6326140
  17. Adaptive and nonadaptive consequences of chemical inhibition of intercellular communication.
    Pharmacol Rev. 1984 Jun;36(2 Suppl):137S-144S PMID: 6382351
  18. Modulation of junctional permeability.
    Fed Proc. 1984 Sep;43(12):2692-6 PMID: 6088293
  19. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
  20. Carbon dioxide reversibly abolishes ionic communication between cells of early amphibian embryo.
    Nature. 1977 Nov 3;270(5632):56-7 PMID: 22047
  21. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  22. Permeability of cell junction depends on local cytoplasmic calcium activity.
    Nature. 1975 Mar 20;254(5497):250-2 PMID: 234601
  23. Differences in intracellular location of pp60src in rat and chicken cells transformed by Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4142-6 PMID: 6254012
  24. Junctional intercellular communication pattern of cultured human breast cancer cells.
    Cancer Res. 1979 Nov;39(11):4739-43 PMID: 498101
  25. Junctional intercellular communication and the control of growth.
    Biochim Biophys Acta. 1979 Feb 4;560(1):1-65 PMID: 216404
  26. Activation of calcium-activated, phospholipid-dependent protein kinase (protein kinase C) by new classes of tumor promoters: teleocidin and debromoaplysiatoxin.
    Biochem Biophys Res Commun. 1984 Apr 30;120(2):339-43 PMID: 6233970
  27. Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3514-8 PMID: 6251464
  28. Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4112-5 PMID: 6270659
  29. Rapid and reversible reduction of junctional permeability in cells infected with a temperature-sensitive mutant of avian sarcoma virus.
    J Cell Biol. 1981 Nov;91(2 Pt 1):573-8 PMID: 6273447
  30. Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
    Cell. 1981 Apr;24(1):165-74 PMID: 6263485
  31. Intercellular communication and the control of growth: XI. Alteration of junctional permeability by the src gene in a revertant cell with normal cytoskeleton.
    J Membr Biol. 1984;82(3):207-12 PMID: 6099421
  32. Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus.
    Nature. 1983 Jul 7-13;304(5921):35-9 PMID: 6306471
  33. The role of protein kinase C in cell surface signal transduction and tumour promotion.
    Nature. 1984 Apr 19-25;308(5961):693-8 PMID: 6232463
  34. The difference in contact inhibition of cell replication between normal cells and cells transformed by different carcinogens.
    Proc Natl Acad Sci U S A. 1966 Dec;56(6):1705-11 PMID: 16591408
  35. Computer-based characterization of epidermal growth factor precursor.
    Nature. 1984 Feb 9-15;307(5951):558-60 PMID: 6607417
  36. Cellular oncogenes and retroviruses.
    Annu Rev Biochem. 1983;52:301-54 PMID: 6351725
  37. Lack of intercellular communication between chemically transformed and surrounding nontransformed BALB/c 3T3 cells.
    Cancer Res. 1984 Nov;44(11):5200-3 PMID: 6488180
  38. Phosphorylation of the ribosomal protein S6 is elevated in cells transformed by a variety of tumor viruses.
    J Virol. 1984 Jun;50(3):966-9 PMID: 6328026
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.16.5360
Published
1985-08-00
Pages
5360-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390568
Subset
IM
Grants
NCI NIH HHS · CA21104 · United States
NCI NIH HHS · CA33158 · United States
NIEHS NIH HHS · ESO-19631 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.16.5360 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2