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PMID: 2987872 Published · ppublish English Journal Article

Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha.

Nucleic acids research ·Vol. 13 ·No. 9 ·1985-05-10 ·Pages 3043-62

Janowicz ZA, Eckart MR, Drewke C, Roggenkamp RO, Hollenberg CP, Maat J, Ledeboer AM, Visser C, Verrips CT

Abstract

A gene library from the methanol utilizing yeast Hansenula polymorpha, constructed in a lambda Charon4A vector, was used to clone the gene encoding a key methanol assimilating enzyme, dihydroxyacetone synthase (DHAS) by differential plaque hybridization. The nucleotide sequence of the 2106 bp structural gene and the 5' and 3' non-coding regions was determined. The deduced amino acid sequence of the protein is in agreement with the apparent molecular weight and amino acid composition of the purified protein. The codon bias is not so pronounced as in some Saccharomyces cerevisiae genes.

MeSH Terms
Aldehyde-Ketone Transferases Amino Acid Sequence Ascomycota/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes/metabolism Endonucleases/metabolism Genes Methanol/metabolism Nucleic Acid Hybridization Pichia/enzymology,genetics Protein Biosynthesis RNA, Messenger/analysis Saccharomyces cerevisiae/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic Transferases/genetics
Chemicals
RNA, Messenger Transferases Aldehyde-Ketone Transferases formaldehyde transketolase Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases Methanol
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Janowicz Z A
Eckart M R
Drewke C
Roggenkamp R O
Hollenberg C P
Maat J
Ledeboer A M
Visser C
Verrips C T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.9.3043
Published
1985-05-10
Pages
3043-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341220
Subset
IM
Databases
GENBANK
X02424
External Links
PubMed source DOI: 10.1093/nar/13.9.3043 PMC Full Text View journal details
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