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PMID: 2960976 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8408-12

Krämer A

Abstract

Upon chromatographic fractionation of HeLa cell nuclear extracts, small RNAs of 145 and 66/65 nucleotides, respectively, were detected that are distinct from the abundant small RNAs present in the extract. These RNAs are precipitated by antibodies directed against the trimethylguanosine cap structure, characteristic for small nuclear RNAs (snRNAs) of the U type. The RNAs of 145 and 66/65 nucleotides appear to be associated with at least one of the proteins common to the major small nuclear ribonucleoprotein particles U1 to U6, since they are specifically bound by anti-Sm antibodies. These criteria characterize the RNAs that are 145 and 66/65 nucleotides in length as U-type snRNAs. Upon gel filtration, the RNAs are found within particles of molecular weights approximately equal to 150,000 and 115,000, respectively. The RNA of 145 nucleotides represents a different minor snRNA, designated U11, whereas the RNA of 66/65 nucleotides may correspond to either mammalian U7 or U10 RNA.

MeSH Terms
Antibodies, Monoclonal Chromatography HeLa Cells/analysis Humans Nuclear Proteins/analysis RNA Caps/analysis RNA, Small Nuclear/analysis Ribonucleoproteins/analysis Ribonucleoproteins, Small Nuclear
Chemicals
Antibodies, Monoclonal Nuclear Proteins RNA Caps RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Krämer A
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, Federal Republic of Germany.
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8408
Published
1987-12-00
Pages
8408-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299552
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.23.8408 PMC Full Text View journal details
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